Virtual genome scan: a tool for restriction landmark-based scanning of the human genome.

Date

2001-08-01

Author

Rouillard, JM
Erson Bensan, Ayşe Elif
Kuick, R
Asakawa, J
Wimmer, K
Muleris, M
Petty, EM
Hanash, S

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There is substantial interest in implementing technologies that allow comparisons of whole genomes of individuals and of tissues and cell populations. Restriction landmark genome scanning (RLGS) is a highly resolving gel-based technique in which several thousand fragments in genomic digests are Visualized simultaneously and quantitatively analyzed. The widespread use of RLGS has been hampered by difficulty in deriving sequence information for displayed fragments and a lack of whole-genome sequence-based framework for interpreting RLGS patterns. We have developed informatics tools for comparisons of sample derived RLGS patterns with patterns predicted from the human genome sequence and displayed as Virtual Genome Scans (VGS). The tools developed allow sequence prediction of fragments in RLGS patterns obtained with different restriction enzyme combinations. The utility of VGS is demonstrated by the identification of restriction fragment length polymorphisms, and of amplifications, deletions, and methylation changes in tumor-derived CpG islands and the characterization of an amplified region in a breast tumor that spanned < 230 kb on 17q23.

Subject Keywords

Electrophoresis, Cell, Cloning, Amplification, Neuroblastoma, Gene, Fragments, 2-Dimensional separations, Dna-sequences, Breast-cancer

URI

https://hdl.handle.net/11511/37274

Journal

Genome research

DOI

https://doi.org/10.1101/gr.181601

Collections

Department of Biology, Article

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Citation Formats

J. Rouillard et al., “Virtual genome scan: a tool for restriction landmark-based scanning of the human genome.,” Genome research, pp. 1453–9, 2001, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37274.