A digital microfluidic interface between solid-phase microextraction and liquid chromatography-mass spectrometry

Date

2016-04-29

Author

Choi, Kihwan
Boyacı, Ezel
Kim, Jihye
Seale, Brendon
Barrera-Arbelaez, Luis
Pawliszyn, Janusz
Wheeler, Aaron R.

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We introduce a method to couple solid-phase microextraction (SPME) with HPLC-MS using digital microfluidics (DMF). In the new system, SPME fibers are used to extract analytes from complex sample solutions, after which the analytes are desorbed into solvent droplets in a DMF device. The open geometry of DMF allows straightforward insertion of SPME fibers without requiring a complicated interface, and automated droplet manipulation enables multiplexed processing of the fibers. In contrast to other multiplexed SPME elution interfaces, the low volumes inherent to DMF allow for pre-concentration of analytes prior to analysis. The new SPME-DMF-HPLC-MS method was applied to the quantification of pg/mL-level free steroid hormones in urine. We propose that this new method will be useful for a wide range of applications requiring cleanup and pre-concentration with convenient coupling to high-performance analytical techniques.

Subject Keywords

Organic Chemistry, Analytical Chemistry, Biochemistry, General Medicine

URI

https://hdl.handle.net/11511/37319

Journal

JOURNAL OF CHROMATOGRAPHY A

DOI

https://doi.org/10.1016/j.chroma.2016.03.029

Collections

Department of Chemistry, Article

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Citation Formats

K. Choi et al., “A digital microfluidic interface between solid-phase microextraction and liquid chromatography-mass spectrometry,” JOURNAL OF CHROMATOGRAPHY A, pp. 1–7, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37319.