Polymerization and biosensor application of water soluble peptide-SNS type monomer conjugates

Date

2017-09-21

Author

SÖYLEMEZ, Saniye
YILMAZ, Tugce
BÜBER, Ece
Udum, Yasemin A.
Özçubukçu, Salih
Toppare, Levent Kamil

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

51
views

0
downloads

A simple and efficient approach for the preparation of a biosensing platform was developed based on newly designed peptide-SNS type monomer conjugates. The approach involves the electrochemical polymerization of the peptide-SNS type monomer on the electrode surface. To synthesize the peptide bearing monomers, the SNS-type monomer having a carboxylic acid functional group was anchored to the C-terminal of the peptide by solid phase peptide synthesis via coupling reagents. Utilization of peptides to increase the solubility of the monomers was first investigated in this report. The obtained monomers, soluble in water, were fully characterized by spectral analyses and utilized as matrices for biomolecule attachment. Polymerization of monomers in water has the potential to provide an alternative process for the electrochemical preparation of the polymers in aqueous media, without using any organic solvent. Under the optimized conditions, the biosensor responded to the target analyte, glucose, in a strikingly selective and sensitive manner, and showed promising feasibility for the quantitative analysis of glucose in beverages.

Subject Keywords

Amperometric Glucose Biosensor, Glycol, Combination, Dimerization, Matrix, İmmobilization, Redox mediator, Carbon nanotubes, Conducting polymers, Anchored carboxylic-acid

URI

https://hdl.handle.net/11511/37477

Journal

JOURNAL OF MATERIALS CHEMISTRY B

DOI

https://doi.org/10.1039/c7tb01674c

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

A novel promising biomolecule immobilization matrix: Synthesis of functional benzimidazole containing conducting polymer and its biosensor applications Uzun, Sema Demirci; Unlu, Naime Akbasoglu; Sendur, Merve; Kanik, Fulya Ekiz; TİMUR, SUNA; Toppare, Levent Kamil (2013-12-01) In order to construct a robust covalent binding between biomolecule and immobilization platform in biosensor preparation, a novel functional monomer 4-(4,7-di(thiophen-2-yl)-1H-benzo[d]imidazol-2yl)benzaldehyde (BIBA) was designed and successfully synthesized. After electropolymerization of this monomer, electrochemical and spectroelectrochemical properties were investigated in detail. To fabricate the desired biosensor, glucose oxidase (GOx) was immobilized as a model enzyme on the polymer coated graphite ...
Synthesis and application of poly-SNS-anchored carboxylic acid: a novel functional matrix for biomolecule conjugation Ekiz, Fulya; Oguzkaya, Funda; Akin, Mehriban; TİMUR, SUNA; Tanyeli, Cihangir; Toppare, Levent Kamil (2011-01-01) Here we report the synthesis of a novel conducting polymer and its properties as an immobilization platform for biosensor application. The conducting polymer has functional groups used for the formation of amide bonding with the enzyme immobilized on the polymer surface. After covalent immobilization of glucose oxidase (GOx) on the polymeric matrix, its application for glucose biosensing was investigated in detail. Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and contact angle ...
A benzimidazole-based conducting polymer and a PMMA-clay nanocomposite containing biosensor platform for glucose sensing EMRE, FATMA BİLGE; Kesik, Melis; Kanik, Fulya Ekiz; Akpinar, Hava Zekiye; Aslan-Gurel, Evren; Rossi, Rene M.; Toppare, Levent Kamil (2015-09-01) Development of materials composed of polymer-clay nanocomposites (PCN) and conducting polymers attracts great interest and preferred in various applications. Hereby, polymethylmethacrylate (PMMA) layered silicate nanocomposites were prepared by in-situ suspension polymerization by grafting PMMA with laponite using a suitable grafting agent. The properties of the as-synthesized PCN materials are characterized by differential scanning calorimetry (DSC), thermal gravimetry analysis (TGA) and gel permeation chr...
Polymerization and characterization of 2-Hydroxyethyl acrylate Vargün, Elif; Usanmaz, Ali; Department of Polymer Science and Technology (2003) Poly(2-Hydroxyethyl acrylate), PHEA, is used as hydrophilic polymeric gels which have been studied because of its great importance for agricultural or biomedical applications. Biomedical applications of hydrogels include soft contact lenses, artificial corneas, soft tissue substitutes and burn dressings. In this study, it was aimed to synthesis the polymers with well-defined molecular weights, polydispersities and cahin topologies. Bulk, solution and atom transfer radical polymerization (ATRP) techniques at...
Bioactive Surface Design Based on Functional Composite Electrospun Nanofibers for Biomolecule Immobilization and Biosensor Applications Uzun, Sema Demirci; Kayaci, Fatma; UYAR, Tamer; TİMUR, SUNA; Toppare, Levent Kamil (2014-04-09) The combination of nanomaterials and conducting polymers attracted remarkable attention for development of new immobilization matrices for enzymes. Hereby, an efficient surface design was investigated by modifying the graphite rod electrode surfaces with one-step electrospun nylon 6,6 nanofibers or 4% (w/w) multiwalled carbon nanotubes (MWCNTs) incorporating nylon 6,6 nanofibers (nylon 6,6/4MWCNT). High-resolution transmission electron microscopy study confirmed the successful incorporation of the MWCNTs in...

Citation Formats

S. SÖYLEMEZ, T. YILMAZ, E. BÜBER, Y. A. Udum, S. Özçubukçu, and L. K. Toppare, “Polymerization and biosensor application of water soluble peptide-SNS type monomer conjugates,” JOURNAL OF MATERIALS CHEMISTRY B, pp. 7384–7392, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37477.