Comparison of culture and PCR methods in detection of haemophilus influenzae, streptococcus pneumoniae and moraxella catarrhalis in children with otitis media with effusion Efüzyonlu orta kulak iltihabi olan çocuklarda haemophilus influenzae, streptococcus pneumoniae ve moraxella catarrhalis saptanmasinda, kültür ve pzr yöntemlerinin karsilastirilmasi

Date

2013-01-01

Author

Baysallar, Mehmet
Güçlü, Aylin Üsküdar
Yetiser, Sertaç
Kiliç, Abdullah
Gözen, Ayşe Gül
Açikel, Cengizhan

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

162
views

0
downloads

The etiology and pathogenesis of otitis media with effusion (OME) is still unclear despite many studies within the last four decades. Polymerase chain reaction (PCR) based procedures are suggested for detection of the causative bacteria supposed to inflict multiple infections. In the current study, culture and PCR based approaches were used to detect the frequency of Haemophilus influenzae, Streptococcus pneumoniae and Moraxella catarrhalis, which have been known as common pathogens in middle ear effusions (MEE) of patients with otitis media. Material and Methods: The DNAs of these three bacteria were detected by standard and multiplex PCR techniques in MEE specimens and their diagnostic values were evaluated in comparison to the conventional culture method. Results: Samples from 67 OME suspected children were analysed retrospectively. Two H. influenzae and two M. catarrhalis isolates were recovered by conventional culture method (6.0%; 4/67). Out of the 67 samples, seven S. pneumoniae, nine H. influenzae, and eleven M. catarrhalis isolates were detected vith PCR. In five samples, two concurrent bacteria were detected in following combinations: two S. pneumoniae and H. influenzae, two S. pneumoniae and M. catarrhalis, and one H. influenzae and M. catarrhalis. Sensitivity, specificity, positive predictive value and negative predictive value rates of the PCR technique were 100.0%, 71.4%, 18.2% and 100.0%, respectively. The difference between culture and PCR was statistically significant (p<0.001). Conclusion: Although the specificity and positive predictive values are low, PCR, which allows rapid screening is feasible for detecting the most common fastidious bacteria that lead to OME.

Subject Keywords

Otitis media with effusion, Culture, Haemophilus influenzae, Streptococcus pneumoniae, Moraxella catarrhalis, Polymerase chain reaction

URI

https://hdl.handle.net/11511/37515

Journal

Turkiye Klinikleri Journal of Medical Sciences

DOI

https://doi.org/10.5336/medsci.2012-28205

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Polymerase chain reaction vs. conventional culture in detection of bacterial pneumonia agents Guclu, AU; Baysallar, M; Gözen, Ayşe Gül; Kilic, A; Balkan, A; Doganci, L (2005-01-01) Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis are important aetiologies of community-acquired pneumonia (CAP). The rapid laboratory diagnosis of CAP is difficult by conventional culture techniques. Polymerase chain reaction (PCR) is an advanced tool, which may solve this problem. Sputum samples were selected and scored by Gram stain. Conventional identification procedures and PCR analysis were applied to the samples and results were compared statistically. Ranging between the a...
Tamoxifen Increases Membrane Fluidity at High Concentrations Severcan, Feride; Kazanci, Nadide; Zorlu, Faruk (Portland Press Ltd., 2000-6-1) There are contradictory results in the literature relating to the effect of tamoxifen on membrane fluidity. The present work investigates the effect of tamoxifen on membrane dynamics to find out whether the concentration of tamoxifen can be one of the factors in this discrepancy. Turbidity (absorbance at 440 nm) and Fourier transform infrared spectroscopic studies reveal that tamoxifen causes opposite effects on membrane fluidity at low (1 mol.%) and high (30 mol.%) tamoxifen concentrations. Low tamoxifen c...
Modulation of Matrix Softness and Interstitial Flow for 3D Cell Culture Using a Cell-Microenvironment-on-a-Chip System Clay, Nicholas Edwin; Shin, Kyeonggon; Özçelikkale, Altuğ; Lee, Min Kyung; Rich, Max H.; Kim, Dong Hyun; Han, Bumsoo; Kong, Hyunjoon (2016-11-01) In the past several decades, significant efforts have been devoted to recapitulating the in vivo tissue microenvironment within an in vitro platform. However, it is still challenging to recreate de novo tissue with physiologically relevant matrix properties and fluid flow. To this end, this study demonstrates a method to independently tailor matrix stiffness and interstitial fluid flow using a cell-microenvironment-on-a-chip (CMOC) platform. Collagen-polyethylene glycol gels tailored to present controlled s...
Characterization and Evaluation of Triamcinolone, Raloxifene, and Their Dual-Loaded Microspheres as Prospective Local Treatment System in Rheumatic Rat Joints Ocal, Yigit; Kurum, Baris; Karahan, Siyami; Tezcaner, Ayşen; Ozen, Seza; Keskin, Dilek (Elsevier BV, 2014-8) In this study, injectable microspheres were developed for the local treatment of joint degeneration in rheumatoid arthritis (RA). Microspheres loaded with triamcinolone (TA), a corticosteroid drug, and/or raloxifene (Ral), a cartilage regenerative drug, were prepared with a biodegradable and biocompatible polymer, polycaprolactone (PCL). Microspheres were optimized for particle size, structural properties, drug release, and loading properties. In vitro release of Ral was very slow because of the low solubil...
Effect of vitamin C and lipoic acid on streptozotocin-induced diabetes gene expression: mRNA and protein expressions of Cu-ZnSOD and catalase Sadi, Goekhan; Yilmaz, Oekkes; Güray, Nülüfer Tülün (Springer Science and Business Media LLC, 2008-02-01) The involvement of oxidative stress in the pathogenesis of diabetes mellitus has been confirmed by numerous studies. In this study, the expression of two antioxidant enzymes, superoxide dismutase (SOD), and catalase which are involved in the detoxification of reactive oxygen species was studied in the streptozotocin-induced diabetic rat liver tissues. The enzyme assays showed a significant decrease in both enzymes activities compared to control animals. The RT-PCR and Western-blot analysis results demonstra...

Citation Formats

M. Baysallar, A. Ü. Güçlü, S. Yetiser, A. Kiliç, A. G. Gözen, and C. Açikel, “Comparison of culture and PCR methods in detection of haemophilus influenzae, streptococcus pneumoniae and moraxella catarrhalis in children with otitis media with effusion Efüzyonlu orta kulak iltihabi olan çocuklarda haemophilus influenzae, streptococcus pneumoniae ve moraxella catarrhalis saptanmasinda, kültür ve pzr yöntemlerinin karsilastirilmasi,” Turkiye Klinikleri Journal of Medical Sciences, pp. 54–58, 2013, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37515.