Evaluating fungal co-production of cellulase and xylanase enzymes at shake-flask scale using distillers dried grains with solubles (DDGS) and its validation in benchtop fermenters

2018-01-01
Most of the corn ethanol plants use dry-mill process and generates large volume of distillers dried grains with solubles (DDGS) as the co-product in addition to ethanol and carbon dioxide. The DDGS is a promising feedstock for fermentation industry due to rich content of cellulose and xylan. Thus, the goal of this study was to optimize production of cellulase and xylanase enzymes by Trichoderma reesei (NRRL 6156) using DDGS as the carbon source at shake-flask scale and validate the shake-flask results at benchtop fermenters. The dilute-acid pretreated DDGS media containing varied levels of DDGS, yeast extract, and peptone were examined for maximal enzyme production at shake-flask at 30oC, initial pH of 5.0 and 180 rpm with 3% (v/v) inoculum size using the Box-Behnken response surface methodology. According to the analyses of results, co-production of xylanase and cellulase enzymes was not achieved in the same medium; higher solid load of DDGS favored cellulase enzyme only while lower solid load induced the xylanase enzyme. Maximal xylanase of 18.5 IU/mL was predicted with 5% DDGS load, 0.1 g/L yeast extract, and 2 g/L peptone, while maximal cellulase of 1.1 IU/mL was predicted with 20% DDGS load, 1 g/L yeast extract, and 0.5 g/L peptone. Of the two developed models obtained from shake-flasks, xylanase model predicted successfully the fermenter results with 1.5 L working volume compared to cellulase model, which needs further refinement., Xylanases, Trichoderma reesei, Submerged fermentation, Cellulases, DDGS,

Suggestions

Optimization of the fermentation parameters to maximize the production of cellulases and xylanases using DDGS as the main feedstock in stirred tank bioreactors
Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (2022-01-01)
Lignocellulolytic enzymes such as cellulases and xylanases are needed on the industrial scales for low cost production of biofuels and the other value-added products from lignocellulosic biomass such as distillers' dried grains with solubles (DDGS). Optimization of fermentation variables such as agitation, aeration, and inoculum size for fungal enzyme production by submerged fermentation can enhance the enzyme production levels. Therefore, this research focuses on the statistical optimization of fungal inoc...
Optimization of the fermentation parameters to maximize the production of cellulases and xylanases using DDGS as the main feedstock in stirred tank bioreactors
Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (2022-10-01)
© 2022 Elsevier LtdLignocellulolytic enzymes such as cellulase and xylanases are needed on the industrial scales for low-cost production of biofuels and the other value-added products from lignocellulosic biomass such as distillers' dried grains with solubles (DDGS). Optimization of fermentation variables such as agitation, aeration, and inoculum size for fungal enzyme production by submerged fermentation can enhance the enzyme production levels. Therefore, this research focused on the statistical optimizat...
The effect of Dilution, Aeration, and Agitation on Fungal Cellulase and Xylanase Production by DDGS-based Fermentation Media in Stirred Tank Bioreactors
Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (2022-01-01)
Fungal cellulases and xylanases are commonly used for biological pretreatment of lignocellulosic biomass into simple sugars, which can then be converted into many value-added products including bioethanol. However, these enzymes are costly to produce and need a feedstock that should be more economical and high in fiber and other nutrients. Distillers dried grains with solubles (DDGS) is the byproduct of corn ethanol production and can be considered an appropriate feedstock. Among various problems in adaptat...
Optimization of nitrogen sources for the maximum production of cellulases and xylanases by Aspergillus Niger strains
Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (2021-01-01)
© ASABE 2021. All rights reserved.Hydrolytic enzymes such as cellulases and xylanases are required for second-generation bioethanol production. Distillers Dried Grains with Solubles (DDGS) has high fiber content which can be used to produce such enzymes by various fungal species. Aspergillus niger is one of the top producers of hydrolytic enzymes. The addition of nitrogen sources can further enhance enzyme production in DDGS based media. Therefore, in this study, two A. niger strains, NRRL 330 and NRRL 567,...
Analysis of pre-service science teachers’ systems thinking skills in the context of carbon cycle
Turan, Deniz; Öztekin, Ceren; Department of Elementary Science and Mathematics Education (2019)
The main purpose of this study is to analyze pre-service science teachers’ systems thinking skills in the context of carbon cycle. The eight systems thinking skills that is previously defined by Ben-Zvi Assaraf and Orion (2005) were used to investigate the participants’ systems thinking skills concerning the carbon cycle. Data was collected from four senior-year pre-service science teachers from elementary science education department of a state university. The data obtained through word association test, c...
Citation Formats
D. Çekmecelioğlu, “Evaluating fungal co-production of cellulase and xylanase enzymes at shake-flask scale using distillers dried grains with solubles (DDGS) and its validation in benchtop fermenters,” 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37637.