Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Nanocrystalline Zn2+ and SO42- binary doped fluorohydroxyapatite: A novel biomaterial with enhanced osteoconductive and osteoinconductive properties
Date
2019-11-01
Author
ALSHEMARY, AMMAR ZEIDAN GHAILAN
Pazarçeviren, Ahmet Engin
Dalgıç, Ali Deniz
Tezcaner, Ayşen
Keskin, Dilek
Evis, Zafer
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
165
views
0
downloads
Cite This
In this study, we have successfully doped hydroxyapatite (HA) with zinc (Zn2+), sulphate (SO42-) and fluoride (F-) ions to develop a new composition of bioceramic, Ca10-xZnx(PO4)(6-y)(SO4)(y)(OH)(2-z-y)F-z(SO4)(y), (x = 0, 0.2, 0.6, 1.0, y = 0, 0.5 and z = 0,1.0 mol), using wet precipitation method. The obtained materials were analysed using XRD, FTIR, FESEM, and XPS techniques to investigate the phase purity, particle morphology and elemental composition, respectively. A model anticancer drug (Doxorubicin, DOX) was loaded onto the surface of the Zn/SO4-FHA materials. About 100% loading of DOX with a controlled release profile was obtained. Degradation of materials in Simulated body fluid (SBF) was greatly improved with the incorporation of Zn2+/SO42- ions in comparison to HA/FHA, which makes it highly bioactive materials. In vitro cell viability and adhesion of Human fetal osteoblast (hFOB) cell were investigated. Cell viability has demonstrated that the hFOB cells proliferated at a high rate on Zn/SO4-FHA materials, confirming the in vitro biocompatibility of the materials. Alkaline phosphatase (ALP) activity and intracellular calcium deposition of hFOB cells seeded on 1ZnSO(4)-FHA disc surface was statistically higher than observed on pure HA and FHA discs, indicating that hFOB cells differentiated into mature osteoblasts on 1Zn/SO4-FHA disc surfaces. Taken together, our results suggest that HA substituted by (Zn2+, 0.2 mol), (SO42-, 0.5 mol) and (F-, 1 mol) (1Zn/So(4)-FHA) material was a promising material for hard tissue scaffolds.
Subject Keywords
Substituted hydroxyapatite
,
Structural characterization
,
Drug delivery
,
In vitro bioactivity
,
Cell proliferation
,
Osteogenic differentiation
URI
https://hdl.handle.net/11511/37844
Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
DOI
https://doi.org/10.1016/j.msec.2019.109884
Collections
Department of Engineering Sciences, Article
Suggestions
OpenMETU
Core
Nano calcium phosphates doped with titanium and fluoride ions: sinterability and stability of phases
Güngör Geridönmez, Serap; Evis, Zafer; Department of Engineering Sciences (2012)
The purpose of this study was to synthesize calcium phosphates doped with titanium and fluoride ions in different combinations. Pure and doped calcium phosphates were synthesized by a precipitation method. The synthesized materials were sintered at 1100ºC and 1300ºC for 1h. The ceramics were characterized by density measurements to determine the effect of sintering temperature. Presence of phases and bonds were characterized by XRD diffraction and FTIR spectroscopy. Grain sizes of the samples were obtained ...
Lithocholic acid conjugated mPEG-b-PCL micelles for pH responsive delivery to breast cancer cells
Isik, Gulhan; Kızıltay, Aysel; Hasırcı, Nesrin; Tezcaner, Ayşen (2022-06-10)
© 2022 Elsevier B.V.In this study, micelles composed of methoxy poly(ethylene glycol)-block-poly(ε-caprolactone) copolymer (mPEG-b-PCL), which has ionically conjugated lithocholic acid (LCA) and providing pH sensitive release of LCA in acidic media, were prepared as drug carrier devices for cancer therapy. Micelles were produced by co-solvent evaporation method at two different temperatures (60 °C and 25 °C) and coded as LCA60**M and LCA25**M, respectively). Hydrodynamic diameters were 86.9 nm and 228.2 nm,...
Folic acid functionalized PEG coated magnetic nanoparticles for targeting anti-cancer drug delivery: Preparation, characterization and cytotoxicity on Doxorubicin, Zoledronic acid and Paclitaxel resistant MCF-7 breast cancer cell lines
Keskin, Tugba; Yalcin, Serap; Gündüz, Ufuk (2018-01-01)
In this study, MNPs were synthesized, coated with biocompatible polyethylene glycol (PEG) and conjugated with folic acid. Crystal and chemical structures, shape, size and magnetic properties of synthesized nanoparticles were characterized. Agglomeration tendency of naked nanoparticles were prevented by oleic acid addition during the synthesis. All synthesized MNPs have been found to exhibit superparamagnetic behavior at 23 degrees C and 37 degrees C. Cytotoxic effects of MNPs were investigated on Doxorubici...
Optimisation of micro-arc oxidation electrolyte for fabrication of antibacterial coating on titanium
Aydogan, Dilek Teker; Muhaffel, Faiz; Kilic, Meryem Menekse; Acar, Ozge Karabiyik; Cempura, Grzegorz; Baydoğan, Murat; Gül-karagüler, Nevin; Kose, Gamze Torun; Czyrska-Filemonowicz, Aleksandra; Çimenoğlu, Hüseyin (2018-01-01)
This study has been carried out to optimise the silver (Ag) content of the coating synthesised on commercially pure titanium (Cp-Ti, Grade 4) for biomedical applications by micro-arc oxidation (MAO) process. The MAO process has been conducted in electrolytes containing silver acetate (AgC2H3O2) at different concentrations between 0 and 0.002 mol L-1. When compared to the base electrolyte, coatings synthesised in >= 0.001 mol L-1 AgC2H3O2 added electrolytes exhibited an antibacterial efficiency of 99.8% agai...
Polyhydroxybutyrate-Coated Magnetic Nanoparticles for Doxorubicin Delivery: Cytotoxic Effect Against Doxorubicin-Resistant Breast Cancer Cell Line
Yalcin, Serap; Unsoy, Gozde; Mutlu, Pelin; Khodadust, Rouhollah; Gündüz, Ufuk (2014-11-01)
In this study, polyhydroxybutyrate (PHB)-coated magnetic nanoparticles (MNPs) were prepared by coprecipitation of iron salts (Fe2+ and Fe3+) by ammonium hydroxide. Characterizations of PHB-coated MNPs were performed by Fourier transform infrared spectroscopy, x-ray diffraction, dynamic light scattering, thermal gravimetric analysis, vibrating sample magnetometry, and transmission electron microscopy analyses. Doxorubicin was loaded onto PHB-MNPs, and the release efficiencies at different pHs were studied un...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
A. Z. G. ALSHEMARY, A. E. Pazarçeviren, A. D. Dalgıç, A. Tezcaner, D. Keskin, and Z. Evis, “Nanocrystalline Zn2+ and SO42- binary doped fluorohydroxyapatite: A novel biomaterial with enhanced osteoconductive and osteoinconductive properties,”
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
, pp. 0–0, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37844.