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Parametric continuous feed stream design to fine-tune fed-batch bioreactor performance: recombinant human growth hormone production in Bacillus subtilis

2016-11-01
ÇALIK GARCİA, GÜZİDE
KOCABAŞ, PINAR
Afsar, Hande
Çalık, Pınar
Ozdamar, Tuncer H.
BACKGROUND: Design principles of a fed-batch bioreactor system to fine-tune bioreactor performance by increasing production and productivity are investigated. To enhance the production and productivity in fed-batch fermentation of recombinant human growth hormone (rhGH) by Bacillus subtilis, this work develops a continuous feed stream (CFS) design method with the parameters, cultivation time to switch batch-to fed-batch operation, so called transition time, t(T), and specific growth rate, mu.