Light-directed functionalization methods for high-resolution optical fiber based biosensors

Date

2015-01-01

Author

Kahyaoğlu, Leyla Nesrin
Stensberg, Matthew
Rickus, Jenna L.

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Recent advances in miniaturization and analyte-sensitive fluorescent indicators make optical fiber biosensors promising alternatives to microelectrodes. Optical sensing offers several advantages over electrochemical methods including increased stability and better spatial control to monitor physiological processes at cellular resolutions. The distal end of an optical fiber can be functionalized with different fluorophore/polymer combinations through mechanical, dip-coating or photopolymerization techniques. Unlike mechanical and dip-coating schemes, photopolymerization can spatially confine the sensing layer in the vicinity of light in a more reproducible and controllable manner. The objective of this study was to fabricate microscale fluorescence lifetime based optrodes using UV-induced photopolymerization. Six commercially available acrylate based monomers were investigated for stable entrapment of the oxygen sensitive porphyrin dye (PtTFPP) dye via photopolymerization at the end of optical fibers. Of these, the acrylate-functionalized alkoxysilane monomer, 3-methacryloxypropyl-trimethoxysilane (tradename Dynasylan MEMO) showed maximal response to changes in oxygen concentration. Dye-doped polymer microtips were grown at the ends 50 mu m optical fibers and sensitivity and response time were optimized by varying both the concentration of doped dye and the excitation power used for polymerization. The resulting sensors showed linear response within the physiologically relevant range of oxygen concentrations and fast response times. While applied here to oxygen sensing, the photopolymer formulation and process parameters described are compatible with a wide range of available organic dyes and can be used to pattern arrays of spots, needles or more complex shapes at high spatial resolution.

Subject Keywords

Optrode, PtTFPP, Oxygen, Photopolymerization, Stern-Volmer, Frequency domain, Fluorescence lifetime, Spatial control

URI

https://hdl.handle.net/11511/38030

DOI

https://doi.org/10.1117/12.2177178

Collections

Department of Food Engineering, Conference / Seminar

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Citation Formats

L. N. Kahyaoğlu, M. Stensberg, and J. L. Rickus, “Light-directed functionalization methods for high-resolution optical fiber based biosensors,” 2015, vol. 9486, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/38030.