DNA aptamer-based colorimetric detection platform for Salmonella Enteritidis

2017-12-15
BAYRAÇ, CEREN
EYİDOĞAN, FÜSUN
Öktem, Hüseyin Avni
Food safety is a major issue to protect public health and a key challenge is to find detection methods for identification of hazards in food. Food borne infections affects millions of people each year and among pathogens, Salmonella Enteritidis is most widely found bacteria causing food borne diseases. Therefore, simple, rapid, and specific detection methods are needed for food safety. In this study, we demonstrated the selection of DNA aptamers with high affinity and specificity against S. Enteritidis via Cell Systematic Evolution of Ligands by Exponential Enrichment (Cell-SELEX) and development of sandwich type aptamer-based colorimetric platforms for its detection. Two highly specific aptamers, crn-1 and crn-2, were developed through 12 rounds of selection with K-d of 0.971 mu M and 0.309 mu M, respectively. Both aptamers were used to construct sandwich type capillary detection platforms. With the detection limit of 103 CFU/mL, crn-1 and crn-2 based platforms detected target bacteria specifically based on color change. This platform is also suitable for detection of S. Enteritidis in complex food matrix. Thus, this is the first to demonstrate use of Salmonella aptamers for development of the colorimetric aptamer-based detection platform in its identification and detection with naked eye in point-of-care.
BIOSENSORS & BIOELECTRONICS

Suggestions

Virulence and antimicrobial resistance genes transfer mechanism between Salmonella Strains by means of Salmonella Genomic Island 1
Namlı, Şahin; Soyer, Yeşim; Department of Food Engineering (2018)
Salmonella, is responsible for significant part of the food borne diseases around the world and isolated abundantly from the food samples. In recent years, with the increasing trend of multidrug-resistance, Salmonella become an important threat for public health. Drug resistance leads inefficiency of antibiotic treatment and it is seemed as a very important problem worldwide as well for Turkey. Drug resistance acceptance mechanism has not been understood yet especially for Salmonella. Salmonella Genomic Isl...
The Contribution of Food Safety Management Systems to the Prevention of Deliberate Food Contamination
Smith, Madeleine (2008-09-20)
Food Safety Management systems such as HACCP can help to prevent deliberate contamination of food. These systems are widely used already by the food industry to prevent accidental contamination. This makes them an attractive and cost effective way to address the issue of deliberate contamination.
Use of Fourier transform infrared spectroscopy for rapid comparative analysis of Bacillus and Micrococcus isolates
Garip, Sebnem; Gözen, Ayşe Gül; Severcan, FERİDE (Elsevier BV, 2009-04-15)
The identification of foodborne microorganisms and their endospores in food products are important for food safety. The present work compares Bacillus (Bacillas licheniformis, Bacillus circulans and Bacillus silbtilis) and Micrococcus (Micrococcus luteus) species with Fourier transform infrared (FTIR) spectroscopy. Our results show that there are several characteristic peaks belonging to both the Micrococcus and Bacillus Species Which Call be used for the identification of these foodborne bacteria and their...
Development of nucleic acid based lateral flow immunochromatographic test platform for salmonella detection
Bulut, Onur; Öktem, Hüseyin Avni; Department of Biology (2014)
Foodborne diseases have been a crucial problem for the public health. Various agents transmitted by food cause these diseases. However, Salmonella accounts for most of the cases leading to most of the hospitalization and even death. Therefore, rapid detection of Salmonella is a considerable step in order to improve food safety and minimize outbreaks. Nucleic acid based biosensors are fast, simple, economic, easy-to-use and do not require trained personnel or high-cost equipments when compared to the current...
Detection of gamma irradiated spices with OSL method and its reliability
AYGÜN, ŞÜKRAN GİZEM; BOZOĞLU, TAHSİN FARUK; Bulur, Enver (2016-01-01)
Irradiation has been accepted as an effective food safety method for various foods over 50 years. Gamma rays from radioactive isotopes of Cobalt 60 or Cesium 137 are used for food irradiation applications. The main concern about food irradiation is the detection of these irradiated foods and also loss of detection of irradiation through the storage period. Photo Stimulated Luminescence (PSL) is one of the physical techniques that can be used for irradiation detection. The aim of this study is to analyze the...
Citation Formats
C. BAYRAÇ, F. EYİDOĞAN, and H. A. Öktem, “DNA aptamer-based colorimetric detection platform for Salmonella Enteritidis,” BIOSENSORS & BIOELECTRONICS, pp. 22–28, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/39003.