Soytaş, Uğur
Aflatoxins B-1 (AFB(1)) and B-2 (AFB(2)) and G(1) and G(2) remain the top mycotoxins routinely analyzed and monitored by Association of Southeast Asian Nations (ASEAN) national laboratories primarily for food safety regulation in the major food commodities, nuts and spices. LC tandem fluorescence detection (LC fluorescence) represents a current mainstream analytical method, with a progressive migration to a primary method by LC tandem MS (MS/MS) for the next half decade. Annual proficiency testing (PT) is conducted by ASEAN Food Reference Laboratories (AFRLs) for mycotoxin testing as part of capability building in national laboratories, with the scope of PT materials spanning from naturally mycotoxin-contaminated spices and nuts in the early 2010s to the recent contamination of corn flour in 2017 for total aflatoxin assay development. The merits of the mainstream LC-fluorescence method are witnessed by a significant improvement (P < 0.05) in PT z-score passing rates (<= 2) from 11.8 to 79.2% for AFB(1), 23.5 to 83.3% for AFB(2), and 23.5 to 79.2% for total aflatoxins in the last 5 years. This paper discusses the journey of ASEAN national laboratories in analytical testing through AFRLs, and the progressive collective adoption of a multimycotoxin LC-MS/MS method aided by an isotopic dilution assay as a future primary method for safer food commodities.


Use of Fourier transform infrared spectroscopy for rapid comparative analysis of Bacillus and Micrococcus isolates
Garip, Sebnem; Gözen, Ayşe Gül; Severcan, FERİDE (Elsevier BV, 2009-04-15)
The identification of foodborne microorganisms and their endospores in food products are important for food safety. The present work compares Bacillus (Bacillas licheniformis, Bacillus circulans and Bacillus silbtilis) and Micrococcus (Micrococcus luteus) species with Fourier transform infrared (FTIR) spectroscopy. Our results show that there are several characteristic peaks belonging to both the Micrococcus and Bacillus Species Which Call be used for the identification of these foodborne bacteria and their...
Analysis of Deoxynivalenol, Zearalenone, T-2, and HT-2 Toxins in Animal Feed by LC/MS/MS-A Critical Comparison of Immunoaffinity Column Cleanup with No Cleanup
Senyuva, Hamide Z.; Gilbert, John; Turkoz, Gozde; Leeman, David; Donnelly, Carol (Oxford University Press (OUP), 2012-11-01)
A comparison has been made of an LC/MS/MS method using direct analysis of acetonitrile extracts of feed and cereal samples and a method using acetonitrile extraction and subsequent immunoaffinity column (IAC) cleanup. Naturally contaminated samples containing one or more of deoxynivalenol, zearalenone, T-2, and HT-2 toxins were analyzed together with test materials containing known toxin levels. LC/MS/MS ion ratios and peak profiles, repeatability, and LOQs were used as the basis for comparing the two appro...
Eriochrome Black T-Eu3+ Complex as a Ratiometric Colorimetric and Fluorescent Probe for the Detection of Dipicolinic Acid, a Biomarker of Bacterial Spores
Yilmaz, M. Deniz; Öktem, Hüseyin Avni (American Chemical Society (ACS), 2018-03-20)
A novel ratiometric colorimetric and fluorescent dual probe based on Eriochrome Black T (EBT) Eu3+ complex was designed to detect dipicolinic acid (DPA), a major constituent of bacterial spores, with high sensitivity and selectivity. UV vis titration experiments demonstrated that EBT and Eu3+ ions formed a 1:1 coordination pair in water. In the presence of Eu3+ ions, the blue solution of EBT changed to magenta, however, upon the addition of DPA, the magenta color changed to blue immediately and characterist...
Immunoaffinity Column Cleanup with Liquid Chromatography Using Postcolumn Bromination for the Determination of Aflatoxins in Black and White Sesame Seed: Single-Laboratory Validation
Liu, Guihua; Zhu, Zhou; Cheng, Jinquan; Senyuva, Hamide Z. (Oxford University Press (OUP), 2012-01-01)
A single-laboratory validation was conducted to establish the effectiveness of an immunoaffinity column cleanup procedure followed by LC with fluorescence detection for the determination of aflatoxins B-1, B-2, G(1), and G(2) in sesame seeds. The sample is homogenized with 50% water (w/w) to form a slurry, then the test portion is extracted with methanol-water (60 + 40, v/v) using a high-speed blender. The sample extract is filtered, diluted with 15% Tween 20 in phosphate-buffered saline solution, and appli...
Microwave assisted extraction of phenolic compounds from tomato and sour cherry pomaces
Şimşek, Meriç; Şümnü, Servet Gülüm; Department of Food Engineering (2010)
The objective of this study was to compare microwave assisted extraction (MAE) and conventional extraction methods for the extraction of total phenolic compounds (TPC) from tomato and sour cherry pomace. Antiradical efficiency (AE) of the extracts and also the concentration of phenolic compounds were determined. In MAE, the effects of microwave power (400 and 700 W), solvent type (water, ethanol and ethanol-water mixture at 1:1 v/v), extraction time (8-20 min) and solvent to solid ratio (10, 20 and 30 ml/g)...
Citation Formats
B. M. ORDU and U. Soytaş, “COMMODITIES DO NOT SHIELD FROM RISK IN FINANCIAL MARKETS, ANYMORE,” 2017, Accessed: 00, 2020. [Online]. Available: