Advancements in non-invasive biological surface sampling and emerging applications

Nalbant, Atakan Arda
Boyacı, Ezel
Biological surfaces such as skin and ocular surface provide a plethora of information about the underlying biological activity of living organisms. However, they pose unique problems arising from their innate complexity, constant exposure of the surface to the surrounding elements, and the general requirement of any sampling method to be as minimally invasive as possible. Therefore, it is challenging but also rewarding to develop novel analytical tools that are suitable for in vivo and in situ sampling from biological surfaces. In this context, wearable extraction devices including passive samplers, extractive patches, and different microextraction technologies come forward as versatile, low-invasive, fast, and reliable sampling and sample preparation tools that are applicable for in vivo and in situ sampling. This review aims to address recent developments in non-invasive in vivo and in situ sampling methods from biological surfaces that introduce new ways and improve upon existing ones. Directions for the development of future technology and potential areas of applications such as clinical, bioanalytical, and doping analyses will also be discussed. These advancements include various types of passive samplers, hydrogels, and polydimethylsiloxane (PDMS) patches/microarrays, and other wearable extraction devices used mainly in skin sampling, among other novel techniques developed for ocular surface and oral tissue/fluid sampling.


Synthesis of Iodo-substituted spiro-fused pyridine derivatives
Teke, Ecem; Zora, Metin; Department of Chemistry (2014)
Spiro cyclic molecules have gained significance because of their biological activities and electronic properties. Spiro frameworks are present in importance natural products and optoelectronic materials. Moreover, due to steric strain, the presence of a spiro carbon atom induces easy rearrangements that can lead to different cyclic products. Although there are some methods to synthesize spiro compounds, the synthesis of iodo-substituted spiro-fused pyridines have not been studied. Accordingly, in this thesis...
Application of in situ Solid-Phase Microextraction on Mediterranean Sponges for Untargeted Exometabolome Screening and Environmental Monitoring
Bojko, Barbara; Onat, Bora; Boyacı, Ezel; Psillakis, Eleftheria; Dailianis, Thanos; Pawliszyn, Janusz (Frontiers Media SA, 2019-10-11)
In marine ecosystems, sponges are ubiquitous sessile organisms that contain a wide range of specialized metabolites. These metabolites point to a diverse range of biochemical pathways. Some of these compounds are biomarkers that indicate the presence of bacteria that form symbiotic relationships with a host sponge (microsymbionts). Sponges hold considerable promise as bioindicators for seawater quality monitoring, as they are exposed to, and accumulate, significant levels of anthropogenic contamination in c...
Improving Current Density Recovery and Magnetic Flux Density Imaging in Diffusion Tensor Magnetic Resonance Electrical Impedance Tomography Applications
Açıkgöz, Berk Can; Eyüboğlu, Behçet Murat; Department of Electrical and Electronics Engineering (2021-8-10)
The electrical conductivity of biological tissues incorporates crucial information regarding the tissues' physiological status. Therefore, there have been many studies for probing the electrical conductivity of biological tissues through Magnetic Resonance Imaging (MRI). Diffusion Tensor Magnetic Resonance Electrical Impedance Tomography (DT-MREIT) is an emerging modality that utilizes a linear relationship between the diffusion tensor and the conductivity tensor and the current density distribution through...
Development of reconstruction algorithms for magnetic resonance-electrical impedance tomography and experimental realization
Birgül, Özlem; Eyüboğlu, Behçet Murat; İder, Y. Ziya; Department of Electrical and Electronics Engineering (2002)
The electrical properties of biological tissues differ among tissues and vary with physiological and pathological state of a solution of bioelectrical field problems. The aim of this study is to reconstruct conductivity images with higher resolution and better accuracy than existing conductivity imaging tech niques. In order to achieve our goal, we proposed a technique named as Mag netic Resonance-Electrical Impedance Tomography (MR-EIT). MR-EIT com bines peripheral voltage measurements of classical Electri...
Investigation of biologically important small molecules: quantum chemical and molecular dynamics calculations
Tekin, Emine Deniz; Erkoç, Şakir; Department of Physics (2010)
In this thesis, six small molecules (S-allylcysteine, S-allyl mercaptocysteine, allicin, methyl propyl disulfide, allyl methyl sulfide and dipropylsulfide) that are found in garlic and onion, and are known to be beneficial for human health were studied using molecular mechanics, semi-empirical methods, ab-initio (Restricted Hartree Fock), and density functional theory. Using the same methods, a synthetic pyrethroid pesticide molecule, called cyfluthrin, was also studied. Structural, vibrational and electron...
Citation Formats
A. A. Nalbant and E. Boyacı, “Advancements in non-invasive biological surface sampling and emerging applications,” Separations, pp. 0–0, 2019, Accessed: 00, 2020. [Online]. Available: