Macrophage-mediated erosion of gamma irradiated poly(trimethylene carbonate) films

Date

2009-08-01

Author

Bat, Erhan
Feijen, Jan
Grijpma, Dirk W.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

77
views

0
downloads

A macrophage culture model was used to investigate the erosion of gamma irradiated poly(trimethylene carbonate) (PTMC) films. When the PTMC films were incubated in the culture medium, but physically separated from the cells by a membrane, no erosion occurred. In contrast, when the J774A macrophages were directly cultured on PTMC films, they adhered to the films and were found to have eroded the polymer surface. Macrophages adhered to gamma irradiated poly(E-caprolactone) (PCL) controls as well, but to a lesser extent than to the PTMC films. In this case, no signs of erosion were observed. Human skin fibroblasts cultured on PTMC and PCL films as controls also adhered to the films but did not erode the surfaces. The effect of enzymes and reactive oxygen species that can be secreted by macrophages on the erosion process was assessed using aqueous solutions of cholesterol esterase, lipoprotein lipase, esterase, potassium superoxide, and hydrogen peroxide. The PTMC films eroded in aqueous enzyme solutions as well as in aqueous superoxide solutions. Cholesterol esterase and superoxide anion radicals seem to be most involved in the macrophage-mediated erosion of PTMC. This macrophage culture model is useful in assessing the influence of macrophages on the in vivo biodegradability of polymers and in elucidating the biodegradation mechanisms involved.

Subject Keywords

Poly(trimethylene carbonate), Macrophages, Enzymatic degradation, Reactive oxygen species, Surface erosion

URI

https://hdl.handle.net/11511/47219

Journal

BIOMATERIALS

DOI

https://doi.org/10.1016/j.biomaterials.2009.03.033

Collections

Department of Chemical Engineering, Article

Suggestions

OpenMETU
Core

Biodegradable Elastomeric Networks: Highly Efficient Cross-Linking of Poly(trimethylene carbonate) by Gamma Irradiation in the Presence of Pentaerythritol Triacrylate Bat, Erhan; Grijpma, Dirk W. (2010-10-01) Biodegradable elastomeric poly(trimethylene carbonate) (PTMC) networks were efficiently formed by gamma irradiating the linear polymer in the presence of pentaerythritol triacrylate (PETA). The properties of networks formed upon irradiation of PTMC films containing (0.1.5 wt %.) PETA as cross-linking aid were evaluated. The gel contents and network densities increased with increasing PETA contents, irradiation dose, and initial polymer molecular weight. At a dose of 25 kGy, networks with gel fractions up to...
Surface characterization and radical decay studies of oxygen plasma-treated PMMA films Ozgen, Ozge; Aksoy, Eda Ayse; Hasırcı, Vasıf Nejat; Hasırcı, Nesrin (2013-04-01) Polymethylmethacrylate (PMMA) films were modified by RF oxygen plasma with various powers applied for different periods, and the effects of these parameters on the surface properties such as hydrophilicity, surface free energy (SFE), chemistry, and topography were investigated by water contact angle, goniometer, X-ray photoelectron spectroscopy (XPS), and atomic force microscopy, and the types of the created free radicals and their decay were detected by electron spin resonance spectroscopy (ESR). SFE and c...
Hydrothermal synthesis of nanostructured TiO2 particles and characterization of their photocatalytic antimicrobial activity ERDURAL, Beril K.; YÜRÜM, Alp; BAKIR, Ufuk; Karakaş, Gürkan (American Scientific Publishers, 2008-02-01) Nanostructured titania particles were synthesized by using hydrothermal processing and the photocatalytic antimicrobial activities were characterized. Both sol-gel synthesized and commercial TiO2 (anatase) samples were processed with two step hydrothermal treatments, under alkaline and neutral conditions. Scanning Electron Microscope (SEM) images showed that alkaline treatment yields nanofibers and lamellar structured particles from the commercial anatase and sol-gel synthesized samples respectively. Furthe...
Microporous vanadosilicate AM-6 films for photocatalytic applications Kuzyaka, Duygu; Akata Kurç, Burcu; Department of Micro and Nanotechnology (2019) There is an increasing attention on the photocatalysis experimentations providing removal of organic contaminants from water under visible and UV radiation. The role of semiconductor materials as photocatalysts is to degrade highly toxic pollutants. Pore regularity, control of defects, and the presence of stoichiometric amounts of vanadium in the silicate framework allows one to create alternative materials in advanced applications such as photocatalysis. Vanadium, present in specific form within the struct...
Acid curing and baking of bastnaesite ore and concentrate Topkaya, Yavuz Ali (1999-01-01) In this study, the hydrometallurgical evaluation of a rare earth ore as well as a concentrate obtained from this ore was done in the laboratory. For the mentioned study, a bastnaesite type rare earth ore located in Beylikahir in Turkey was used. The total rare earth oxide (REO) content of the deposit was estimated to be 1 million tons with an average concentration of 3.42% REO. The rare earths were contained in bastnaesite mineral. The other constituents of the ore were calcium fluoride (52.5%), barite (25....

Citation Formats

E. Bat, J. Feijen, and D. W. Grijpma, “Macrophage-mediated erosion of gamma irradiated poly(trimethylene carbonate) films,” BIOMATERIALS, pp. 3652–3661, 2009, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/47219.