Evaluation of human dental pulp stem cells behavior on a novel nanobiocomposite scaffold prepared for regenerative endodontics

Date

2019-07-01

Author

Rad, Reza Moonesi
Atila, Deniz
Akgun, Elif Ece
Evis, Zafer
Keskin, Dilek
Tezcaner, Ayşen

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

83
views

0
downloads

Dental caries is a dental disease affecting public health, which results in many socio-economic consequences. This disease causes loss of tooth hard tissue and subsequent inflammation and loss of the dental pulp. In this study, it was aimed to develop and characterize boron (B) modified bioactive glass nanoparticles (BG-NPs) containing cellulose acetate/oxidized pullulan/gelatin (CA/ox-PULL/GEL) three dimensional scaffolds with tubular morphology for dentin regeneration. 3D nanobiocomposite structures were prepared by thermally induced phase separation and porogen leaching methods and characterized by in vitro degradation analysis, water absorption (WA) capacity measurement, SEM, in vitro biomineralization analysis, porosity measurement and mechanical tests. Scaffolds lost about (30-40)% of their weight during one month and WA capacity decreased with increase in immersion time in phosphate buffer solution (PBS) during one month. According to SEM, aligned and tubular structures were formed with mean diameter of about 11 mu m, and BG-NPS were distributed evenly in all parts of the scaffolds. Scaffolds (without BG-NPs) possessed the highest porosity percentage. Addition of 10% BG-NPs improved the mechanical properties of scaffolds. Scaffolds surfaces were fully covered by calcium phosphate (Ca-P) deposits after conditioning in simulated body fluid for 14 days with higher quantity of deposition in groups with inclusion of B-BG-NPs. Human dental pulp stem cells (hDPSCs) were isolated from third molar teeth and used in cell culture studies. In all groups, cells adhered well 1 day after culture. Group B14-10 showed a slight increase of proliferation than group (without BG-NPs) after 7 days of incubation. Alkaline phosphatase activity (ALP) and intracellular calcium amounts increased significantly 14 days after incubation with highest values in B14-10 and B14-20 groups. Confocal laser scanning microscopy (CSLM) analysis, showed that cells on B14-10 and 821-10 scaffold groups, spread more 14 days after culture, and they also possessed extended processes specific to odontoblasts. Alizarin Red quantification showed that the highest calcium deposition was observed on B14-10 scaffolds. Immunohistochemical and Von Kossa stainings showed that scaffolds positively affected the odontoblastic differentiation of the hDPSCs. In this work, results showed that boron modified BG-NPs (B-BG-NPs) incorporated dentin-like constructs bring a new approach for dental tissue engineering applications.

Subject Keywords

Mechanical Engineering, General Materials Science, Mechanics of Materials, Condensed Matter Physics

URI

https://hdl.handle.net/11511/47361

Journal

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS

DOI

https://doi.org/10.1016/j.msec.2019.03.022

Collections

Department of Engineering Sciences, Article

Suggestions

OpenMETU
Core

Effect of MgF2 on hot pressed hydroxylapatite and monoclinic zirconia composites Evis, Zafer (Springer Science and Business Media LLC, 2007-06-01) Hydroxylapatite (HA) has been widely used in biomedical applications because of its excellent biocompatibility in the human body. A total of 25 wt% monoclinic (m) zirconia-HA composites (with and without 5 wt% MgF2) were synthesized to investigate their mechanical properties and phase stability. In HA-m-ZrO2 composites, HA and m-ZrO2 reacted to form CaZrO3 when there was no F- present in the composite and M-ZrO2 partially transformed to tetragonal ZrO2. When MgF2 was added into the system, it improved the t...
Comparison of magnetic Barkhausen noise and ultrasonic velocity measurements for microstructure evaluation of SAE 1040 and SAE 4140 steels Gür, Cemil Hakan; Çam, İbrahim (Elsevier BV, 2007-05-01) The aim of this study is to compare the performance of magnetic Barkhausen noise and ultrasonic methods for the evaluation of the microstructure of commercial steels. Following the austenitization of the specimens made of SAE 1040 and SAE 4140, various heat treatments were carried out to obtain microstructures consisting of martensite, tempered martensite, fine pearlite-ferrite, and coarse pearlite-ferrite. The microstructures were initially characterized by SEM investigation and hardness measurements. The ...
Design and mechanical analysis of a new dental implant that would mimic natural tooth with a periodontal ligament Pektaş, Ömer; Tönük, Ergin; Department of Mechanical Engineering (2012) Dental implant is an artificial dental root that is used to construct dental restorations, similar to the original teeth, in order to regain the function of missing teeth of patients experiencing tooth loss. At the interface between the jawbone and the roots of natural teeth, a thin, elastic, shock absorbing tissue, called the periodontal ligament (PDL), forms a cushion which provides certain mobility to the natural teeth. The restorations supported by dental implants, however, involve completely rigid stru...
Predicting clinical outcomes in neuroblastoma with genomic data integration Baali, Ilyes; Acar, D Alp Emre; Aderinwale, Tunde W.; HafezQorani, Saber; Kazan, Hilal (Springer Science and Business Media LLC, 2018-9-27) Background: Neuroblastoma is a heterogeneous disease with diverse clinical outcomes. Current risk group models require improvement as patients within the same risk group can still show variable prognosis. Recently collected genome-wide datasets provide opportunities to infer neuroblastoma subtypes in a more unified way. Within this context, data integration is critical as different molecular characteristics can contain complementary signals. To this end, we utilized the genomic datasets available for the SE...
Effects of hydrogen peroxide bleaching on human dentin and enamel microstructure and function Gökduman, Kurtuluş; Severcan, Feride; Department of Biotechnology (2005) In recent years bleaching of vital teeth has become popular among both dentist and patients. Different bleaching agents were used for this purpose. They are either applied professionally at high dose (office bleaching) or by patient at lower dose (home bleaching). In the present work we studied the effects of a high concentration bleaching agent (35% hydrogen peroxide), and a low concentration bleaching agent (17% carbamide peroxide) on human enamel and dentin using Fourier Transform Infrared (FTIR) Spectro...

Citation Formats

R. M. Rad, D. Atila, E. E. Akgun, Z. Evis, D. Keskin, and A. Tezcaner, “Evaluation of human dental pulp stem cells behavior on a novel nanobiocomposite scaffold prepared for regenerative endodontics,” MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, pp. 928–948, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/47361.