Polyacrylamide-gelatine carrier system used for invertase immobilization

Date

2006-08-01

Author

Emregul, E
Sungur, S
Akbulut, Ural

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Invertase was immobilized into polyacrylamide-gelatin carrier system by chemical cross-linking with chromium (III) acetate, chromium (III) sulphate, and potassium chromium (III) sulphate. The optimum conditions, namely substrate concentration, temperature, and pH were determined. The effect of polyacrylamide-gelatin ratio and cross-linker concentration oil immobilized enzyme activity were analysed. Maximum immobilized enzyme activities were obtained with chromium (III) acetate (0.01 mol dm(-3)), chromium (III) sulphate (0.004 mol dm(-3)) and potassium chromium (III) sulphate (0.001 mol dm(-3)) for 0.177 (w/w) polyacrylamide-gelatin carrier ratio as 79%, 72% and 79%, respectively. The K-m values were 86 and 166 mM for free and immobilized enzyme, respectively. All immobilized samples were used 20 times over a period of 2 months without a considerable loss of activity.

Subject Keywords

Food Science, Analytical Chemistry, General Medicine

URI

https://hdl.handle.net/11511/47818

Journal

FOOD CHEMISTRY

DOI

https://doi.org/10.1016/j.foodchem.2005.05.017

Collections

Department of Chemistry, Article

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Citation Formats

E. Emregul, S. Sungur, and U. Akbulut, “Polyacrylamide-gelatine carrier system used for invertase immobilization,” FOOD CHEMISTRY, pp. 591–597, 2006, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/47818.