Immobilization of invertase in conducting thiophene-capped poly(methylmethacrylate)/polypyrrole matrices

Date

1999-01-01

Author

Alkan, S
Toppare, Levent Kamil
Yagci, Y
Hepuzer, Y

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Immobilization of invertase in thiophene-capped poly(methylmethacrylate)/polypyrrole matrices was achieved by constant potential electrolysis using different supporting electrolytes. Optimum reaction conditions such as substrate concentration, temperature, and pH for the enzyme electrodes were determined. The temperature and pH were found to be 60 degrees C and 4.8, respectively. The effect of supporting electrolyte on the enzyme activity revealed that SDS was the best in the immobilization procedure. Michaelis-Menten constant and the maximum reaction rate in PMMA/PPy matrices were of the order of that of pristine polypyrrole. However, in terms of repeated use, the copolymer matrices were superior to polypyrrole.

Subject Keywords

Biophysics, Bioengineering, Biomaterials, Biomedical Engineering

URI

https://hdl.handle.net/11511/47992

Journal

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION

DOI

https://doi.org/10.1163/156856299x00036

Collections

Department of Chemistry, Article

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Citation Formats

S. Alkan, L. K. Toppare, Y. Yagci, and Y. Hepuzer, “Immobilization of invertase in conducting thiophene-capped poly(methylmethacrylate)/polypyrrole matrices,” JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, pp. 1223–1235, 1999, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/47992.