Synthesis of DNA-encapsulated silica elaborated by sol-gel routes

Kapusuz, Derya
Durucan, Caner
The highly specific functions of DNA can be used for designing novel functional materials. However, aqueous solubility and biochemical instability of DNA impede its direct utilization as a functional component. Herein, preparation of a hybrid material encapsulating the DNA molecules (double-stranded salmon sperm, 50-5000 base pairs) in robust host-sol-gel-derived silica-has been described. The encapsulation was carried out in two steps: hydrolysis of an acidic tetraethylorthosilicate [Si(OC2H5)(4)] sol and was followed by condensation near physiological pH upon addition of alkaline DNA-containing solutions. The gelation behavior and structural properties of the DNA-silica hybrids were investigated by Si-29 nuclear magnetic resonance and by nitrogen adsorption. The selective adsorption of a DNA-interactive reagent molecule (ethidium bromide) in their diluted aqueous solutions on DNA-silica hybrids confirmed that the DNA molecules remained entrapped within the silica host without any deterioration. A DNA encapsulation mechanism correlating the silica microstructure and DNA holding efficiency has been proposed.


Preparation of anti-adhesive and antibacterial surfaces using polymers
Onat, Bora; İErel Göktepe, İrem; Banerjee, Sreeparna; Department of Biotechnology (2017)
Layer-by-layer self-assembly of polymers is a versatile technique which can impart new functions to the surfaces of biomedical instruments and biomaterials. Materials which are coated by this technique can exhibit response towards environmental stimuli, such that the controlled release of drugs and similar biologically functional molecules under different stimuli can be observed. In the span of this thesis study, ultra-thin polymer films were prepared through the layer-by-layer self-assembly technique, the ...
Synthesis of silver nanowires through polyol process
Coşkun, Şahin; Ünalan, Hüsnü Emrah; Department of Metallurgical and Materials Engineering (2012)
Nanotechnology enabled synthesis of various shapes and morphologies of conventional materials. Nanotubes, nanoparticles, quantum dots and nanowires are the new form of materials. Especially nanowires have gotten great attention due to their unique physical, chemical and optical properties. Superior properties of nanowires are based on their high surface area and two quantum confinement directions. Silver is one of the most conductive metals and it has the highest thermal conductivity. Due to excellent prope...
Preparation and characterization of poly(epsilon-caprolactone) scaffolds modified with cell-loaded fibrin gel
Malikmammadov, Elbay; Endoğan Tanır, Tuğba; Kızıltay, Aysel; Hasırcı, Nesrin (2019-03-15)
Poly(epsilon-caprolactone) (PCL) is one of the most commonly used polymers in the production of tissue engineered scaffolds for hard tissue treatments. Incorporation of cells into these scaffolds significantly enhances the healing rate of the tissue. In this study, PCL scaffolds were prepared by wet spinning technique and modified by addition of fibrinogen in order to form a fibrin network between the PCL fibers. By this way, scaffolds would have micro and nanofibers in their structures. Drying of the wet s...
Synthesis of Emulsion-Templated Acrylic-Based Porous Polymers: From Brittle to Elastomeric
Tunc, Yeliz; Hasırcı, Nesrin; ULUBAYRAM, KEZBAN (2012-01-01)
High internal phase emulsion polymers (PolyHIPEs) are novel materials that have high porosity and interconnected open-cell structure and are used in various applications such as supports for catalytic systems, media for separation of similar molecules, and scaffolds for tissue engineering. In this study, 90% porous acrylic based polyHIPE structures with various cellular structure and mechanical characteristics were developed by using stearyl acrylate (SA), isodecyl acrylate (IDA), isobornyl methacrylate (IB...
Synthesis, characterization and osteoblastic activity of polycaprolactone nanofibers coated with biomimetic calcium phosphate
MAVİŞ, BORA; Demirtas, Tolga T.; GÜMÜŞDERELİOĞLU, MENEMŞE; Gündüz, Güngör; Colak, Uner (2009-10-01)
Immersion of electrospun polycaprolactone (PCL) nanofiber mats in calcium phosphate solutions similar to simulated body fluid resulted in deposition of biomimetic calcium phosphate layer on the nanofibers and thus a highly bioactive novel scaffold has been developed for bone tissue engineering. Coatings with adequate integrity, favorable chemistry and morphology were achieved in less than 6 h of immersion. In the coating solutions, use of lower concentrations of phosphate sources with respect to the literat...
Citation Formats
D. Kapusuz and C. Durucan, “Synthesis of DNA-encapsulated silica elaborated by sol-gel routes,” JOURNAL OF MATERIALS RESEARCH, pp. 175–184, 2013, Accessed: 00, 2020. [Online]. Available: