Hybrid fed-batch bioreactor operation design: control of substrate uptake enhances recombinant protein production in high-cell-density fermentations

Date

2018-11-01

Author

Çalık, Pınar
ÇALIK GARCİA, GÜZİDE
Ozdamar, Tuncer H.

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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BACKGROUNDThe hybrid fed-batch bioreactor (HFBB) operation is a novel design method for high-cell-density fermentations. It employs fed-batch and batch operations, starting with fed-batch that in turn shifts to the next, and proceeds in successive cycles to enhance production and productivity by controlling the substrate uptake rate.

Subject Keywords

Hybrid fed-batch, Bioreactor operation, High-cell-density fermentation, Substrate uptake, Fed-batch, Pichia pastoris, GAP promoter

URI

https://hdl.handle.net/11511/48978

Journal

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

DOI

https://doi.org/10.1002/jctb.5696

Collections

Department of Chemical Engineering, Article

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Citation Formats

P. Çalık, G. ÇALIK GARCİA, and T. H. Ozdamar, “Hybrid fed-batch bioreactor operation design: control of substrate uptake enhances recombinant protein production in high-cell-density fermentations,” JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, pp. 3326–3335, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48978.