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Zeolit ince filmlerinin biyosensör uygulamalarına yönelik olarak hazırlanması ve araştırılması

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2012
Zeolites plays an important role in the immobilization of biomolecules with their tunable surface properties, adjustable surface charge and hydrophilicity, which is a function of crystal Si/Al ratio. These properties make zeolites promising alternative candidates for the immobilization of enzymes and incorporation into biosensing devices. The objective of the project is to successfully assemble individual pieces of elements, such as different nanomaterials, biological compounds, and transducers into each other in order to study the role of nanomaterials in the assembled piece of work. For this purpose, zeolites and zeo-type materials with varying characteristics were synthesized; zeolite thin films were made and microfabricated to obtain different zeolite patterns from the synthesized materials; immobilization of biologic compounds were investigated onto these thin films; and finally different types of electrochemical biosensors were fabricated using these individual pieces to obtain actual data out of the integrated devices in order to discuss the role and significance of the nanomaterials for biosensors. During the first phase of the project, micron and nano sized zeolite materials with homogenous particle size and varied Si/Al ratio were synthesized and modified by using different techniques. Throughout the project, all zeolites that were aimed to be produced were successfully synthesized and modified by either applying ionexchange protocols or changing surface groups. During the second phase, zeolite particles were attached onto SiO2 surfaces to obtain zeolite thin films. For this purpose, the quality of the obtained thin films, their potentiality to be used in biosensors and thier use in cell attachement applications were investigated by studying the changing zeolite parameters such as the morphology, varying particle size, changing Si/Al ratio and thus the changing hydrophilic/hydrophobic surface properties of zeolites. The third phase of the project involves immobilization of different biological compounds onto the obtained zeolites and their thin films and then to actually gather biosensor data from the assembled piece of device. Next phase of the project involves obtaining performance characteristics of biosensors such as stability, life time, and inhibition using these assembled pieces. During the last stage of our project, all zeolite thin films that were microfabricated were subjected to cell attachment tests using osteoblasts and fibroblasts and the number of cells were counted using MTT tests. In this way, it was possible to control the number of zeolites in the micropatterns and it was found that this number directly correlated with the number of cells that were attached and their proliferation was investigated as a function of attached zeolites.