Indenoquinoxalinone based conjugated polymer substrate for laccase biosensor

Cevher, Şevki Can
Çırpan, Ali
Bekmezci, Sıddıka Aybüke
Soylemez, Saniye
Udum, Yasemin Arslan
Toppare, Levent Kamil
This paper describes the synthesis and characterization of a novel monomer (6,9-bis(4-hexylthiophen-2-yl)-11H-indeno[2,1-b]quinoxalin-11-one (M1)). M1 monomer was electrochemically polymerized on the electrode surface and used as a sensing platform for the immobilization of laccase. Moreover, electrochemical and optical properties of the corresponding polymer were performed in detail. To the best of our knowledge, electrochemical and biosensor performance of the polymer were investigated for the first time. The PM1/Laccase were used in the fabrication of a new biosensor for determination of catechol in water samples by amperometric methods. Under optimized conditions, the calibration curve showed a linear range of 0.005–0.175 mM, with a limit of detection of 9.86 μM and high sensitivity of 153.6 μA/mMcm2. The biosensor exhibited good stability, selectivity and high sensitivity toward catechol.
Materials Chemistry and Physics


Conducting polymers of decanedioic acid bis-(4-pyrrol-1-yl-phenyl) ester
Çırpan, Ali; Toppare, Levent Kamil (Elsevier BV, 2004-05-15)
A dipyrrolyl monomer was synthesized via the reaction between 4-pyrrol-1-yl phenol and decanedioyl dichloride. The electrochemical behavior of this monomer was studied. Polymerization of decanedioic acid bis-(4-pyrrol-1-yi-phenyl) ester (DAPE) was achieved by chemical and constant current electrolyses methods. Copolymerization of DAPE with thiophene was performed by constant potential electrolysis in acetonitrile-tetrabutylammonium tetrafluoroborate (TBAFB), dichloromethane-TBAFB, solvent-electrolyte couple...
Encapsulated Hydrogels by E-beam Lithography and Their Use in Enzyme Cascade Reactions
Mancini, Rock J.; Paluck, Samantha J.; Bat, Erhan; Maynard, Heather D. (American Chemical Society (ACS), 2016-04-26)
Electron beam (e-beam) lithography was employed to prepare one protein immobilized hydrogel encapsulated inside another by first fabricating protein-reactive hydrogels of orthogonal reactivity and subsequently conjugating the biomolecules. Exposure of thin films of eight arm star poly(ethylene glycol) (PEG) functionalized with biotin (Biotin PEG), alkyne (Alkyne-PEG) or aminooxy (AO-PEG) end groups to e-beam radiation resulted in cross-linked hydrogels with the respective functionality. It was determined vi...
Bromine mediated partial oxidation of ethane over nanostructured zirconia supported metal oxide/bromide
Yılmaz, Ayşen; Zhou, XP; Lorkovic, IM; Ylmaz, GA; Laverman, L; Weiss, M; Sun, SL; Schaefer, D; Sherman, JH; McFarland, EW; Ford, PC; Stucky, GD (Elsevier BV, 2005-04-01)
Partial oxidation of ethane to an array of products was achieved through use of bromine/supported metal bromide as redox mediator for oxygen activation. In a two stage flowing fixed bed reactor, ethane bromination (350-450 degrees C) to EtBr and HBr was followed by metathesis of these intermediates with supported cobalt or copper oxides (200-250 degrees C) to produce ethanol (up to 64% selectivity at 45% EtBr conversion), diethyl ether (64% selectivity at 44% conversion), and ethylene (26% selectivity at 68...
Immobilization of Tyrosinase in Poly(2-thiophen-3-yl-alkyl ester) Derivatives
ÇAMURLU, PINAR; Kayahan, Senem; Toppare, Levent Kamil (Informa UK Limited, 2008-01-01)
In this study, construction of novel biosensors for the determination of phenolic compound was performed via immobilization of tyrosinase during the electrochemical synthesis of conducting block copolymers of 2-thiophen-3-yl-alkyl ester derivatives with 3,4-ethylenedioxythiophene and synthesis of poly(3,4-ethylenedioxythiophene) (PEDOT). The resultant biosensors were characterized in terms of their maximum reaction rates, Michaelis-Menten constants (Km), temperature and pH stabilities. All the copolymer mat...
In Situ Formed "Weakly Ligated/Labile Ligand" Iridium(0) Nanoparticles and Aggregates as Catalysts for the Complete Hydrogenation of Neat Benzene at Room Temperature and Mild Pressures
Bayram, Ercan; Zahmakiran, Mehmet; Özkar, Saim; Finke, Richard G. (American Chemical Society (ACS), 2010-07-20)
"Weakly ligated/labile ligand" nanoparticles, that is nanoparticles where only weakly coordinated ligands plus the desired catalytic reactants are present, are of fundamental interest. Described herein is a catalyst system for benzene hydrogenation to cyclohexane consisting of "weakly ligated/labile ligand" Ir(0) nanoparticles and aggregates plus dry-HCl formed formed in situ from commercially available [(1,5-COD)IrCl](2) plus 40 +/- 1 psig (similar to 2.7 atm) H(2) at 22 +/- 0.1 degrees C. Multiple control...
Citation Formats
Ş. C. Cevher, A. Çırpan, S. A. Bekmezci, S. Soylemez, Y. A. Udum, and L. K. Toppare, “Indenoquinoxalinone based conjugated polymer substrate for laccase biosensor,” Materials Chemistry and Physics, pp. 0–0, 2021, Accessed: 00, 2020. [Online]. Available: