A new electrochemical method for the production of stable ascorbate free radicals

Önal, Ahmet
Öǧüş, Ay
Kısakürek, Duygu
A new method for the production of ascorbate free radicals is established. The radical is produced from ascorbate in deionized water by applying constant potential electrolysis under a nitrogen atmosphere. Prior to electrolysis, a cyclic voltammogram (CV) of the ascorbic acid was obtained. Electrolysis potentials were selected as the oxidation peak potential of the ascorbic acid obtained by CV. The detection of the radical was done by electron spin resonance (esr) and uv spectroscopies.
Journal of Biochemical and Biophysical Methods


A new strategy for the synthesis of pyridines from N-propargylic beta-enaminothiones
Kelgokmen, Yilmaz; Zora, Metin (Royal Society of Chemistry (RSC), 2019-03-07)
A new method for the synthesis of 2,4,5-trisubstituted pyridines from N-propargylic beta-enaminothiones is reported. beta-Enaminothiones were prepared by thionation of the corresponding beta-enaminones with Lawesson's reagent. When treated with diisopropylamine in DMF at room temperature, N-propargylic beta-enaminothiones yielded 2,4,5-trisubstituted pyridines in moderate to high yields, along with small amounts of 2-methylene-2,3-dihydro-1,4-thiazepines. The reaction was found to be general for a broad ran...
A promising enzyme anchoring probe for selective ethanol sensing in beverages
Soylemez, Saniye; Goker, Seza; Toppare, Levent Kamil (Elsevier BV, 2019-07-15)
A newly designed amperometric biosensor for the determination of ethanol through one-step electrochemical coating of (4,7-di(thiophen-2-yl)benzo[c][1,2,5]selenadiazole-co-1H-pyrrole-3-carboxylic acid) (TBeSe-co-P3CA) on a graphite electrode is presented. It was aimed to propose a newly synthesized copolymer with enhanced biosensing properties as a novel sensor for the quantification of ethanol. The conjugated copolymer (TBeSe-co-P3CA) was prepared through electrochemical polymerization by potential cycling....
A probabilistic approach to microRNA-target binding
OĞUL, HASAN; Umu, Sinan U.; Tuncel, Y. Yener; Akkaya, Mahinur (Elsevier BV, 2011-09-16)
Elucidation of microRNA activity is a crucial step in understanding gene regulation. One key problem in this effort is how to model the pairwise interactions of microRNAs with their targets. As this interaction is strongly mediated by their sequences, it is desired to set-up a probabilistic model to explain the binding preferences between a microRNA sequence and the sequence of a putative target. To this end, we introduce a new model of microRNA-target binding, which transforms an aligned duplex to a new se...
A facile synthesis of a novel family of heterotricyclic hybrids: Spiro-pyrrolopyridazines
Dündar, Buse Ayşen; Zora, Metin (2022-01-01)
An efficient strategy for the synthesis of spiro-pyrrolopyridazines has been developed. When reacted with hydrazine monohydrate, spiro-2H-pyrroles with a 1,4-diketone functionality produced a novel family of heterotricyclic hybrids, spiro-pyrrolopyridazines, specifically spiro{cyclohexane-1,5'-pyrrolo[3,4-d]pyridazine} systems. The condensation reaction of spiro-2H-pyrroles with hydrazine monohydrate in refluxing 1-butanol proceeded well and afforded spiro-pyrrolopyridazines in good to high yields. The reac...
An amperometric acetylcholine biosensor based on a conducting polymer
Kanik, Fulya Ekiz; Kolb, Marit; TİMUR, SUNA; Bahadir, Muefit; Toppare, Levent Kamil (Elsevier BV, 2013-08-01)
An amperometric acetylcholine biosensor was prepared by the generation of the conducting polymer poly(4-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl)benzenamine) (poly(SNS-NH2)) on graphite electrodes. For pesticide detection, the enzymes acetylcholinesterase (AChE) and choline oxidase (ChO) were co-immobilized onto the conducting polymer poly(SNS-NH2) films using covalent binding technique. Electrochemical polymerization was carried out using a three-electrode cell configuration via cyclic voltammetry. Characteri...
Citation Formats
A. Önal, A. Öǧüş, and D. Kısakürek, “A new electrochemical method for the production of stable ascorbate free radicals,” Journal of Biochemical and Biophysical Methods, pp. 137–142, 1990, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/51449.