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Attachment and growth kinetics of anchorage-dependent BHK cells on microcarriers

Kiremitci, Mustafa
Özilgen, Mustafa
Pişkin, Erhan
Anchorage-dependent Baby Hamster Kidney (BHK) cells were cultivated on polyhydroxyethylmethacrylate (PHEMA), polystyrene (PS), and Cytodex microcarriers. Analysis of the experimental data indicated that there were a finite number of sites on the microcarrier surfaces, available for anchorage. The number of these sites was determined by the chemical and physical structure of the surface. A small fraction of these sites were suitable for attachment of the cells before proliferation. A larger fraction of these sites did not support attachment but the cells could proliferate on them by the help of previously attached mother cells. The attachment and proliferation of the BHK cells on these microcarriers were satisfactorily modeled by surface saturation type of mathematical expressions.