Cloning and expression studies of putative Rhizopus oryzae hexokinase genes in Saccharomyces cerevisiae

Alagoz, E.
Acar, S.
Hamamcı, Haluk


Cloning of the scytalidium thermophilum bifunctional catalase / phenol oxidase gene and expression in aspergillus sojae
Erçin, Hatice Özlem; Ögel, Zümrüt Begüm; Department of Food Engineering (2008)
Scytalidium thermophilum is a thermophilic fungus with an important role in the composting process of mushroom cultivation. An extracellular phenol oxidase of Scytalidium thermophilum (STEP) with novel features was previously studied in our laboratory. This enzyme later turned out to be a catalase having phenol oxidase activity. The aim of this study was to clone Scytalidium thermophilum bifunctional catalase/phenol oxidase encoding gene and express the gene in Aspergillus sojae for future site directed mut...
Cloning and screening of the putative hexokinase genes from Rhizopus oryzae and their heterologous expression in Saccharomyces cerevisiae
Alagoz, Eda; Acar, Seyda; Yucel, Meral; Hamamcı, Haluk (2022-06-01)
Background A filamentous fungus, Rhizopus oryzae (R. oryzae) is one of the ideal candidates for ethanol and lactic acid production due to its ability to grow on renewable carbon sources.
Cloning, expression and sequencing of citrate synthase from thermoplasma volcanium
Çekiç, Çağlar; Kocabıyık, Semra; Department of Biotechnology (2004)
In this study first time, we have cloned and sequenced the citrate synthase gene from a thermoacidophilic archaeon Thermoplasma (Tp.) volcanium (Optimum growth temperature of Tp.volcanium is 60oC and optimum pH is 2.0.). For cloning we have followed a PCR based approach. Amplification of citrate synthase gene from chromosomal DNA of Tp.volcanium yielded a product of 1476 bp containing an open reading frame of 1161 bp comprising the structural gene. After ligation of the PCR amplicon to pDrive vector through...
Cloning and Expression of a Haloacid Dehalogenase Gene from Pseudomonas sp Strain 19S
Kocabıyık, Semra (null; 1994-07-03)
Cloning and characterization of industrially important alpha-galactosidase genes from the human pathogen aspergillus fumigatus
Söyler, U. Betül; Ögel, Zümrüt Begüm; Department of Food Engineering (2004)
In this study, molecular cloning studies were performed on the a-galactosidase genes of Aspergillus fumigatus IMI 385708. This organism is an opportunistic saprophytic fungus and a human pathogen, mainly affecting immunocompromised patients. A. fumigatus is a thermotolerant fungus and can efficiently produce thermostable a-galactosidase. Two different cloning strategies were undertaken in this study. A. fumigatus cDNA library, prepared previously, was screened with three different probes. No net results wer...
Citation Formats
E. Alagoz, S. Acar, M. YÜCEL, and H. Hamamcı, “Cloning and expression studies of putative Rhizopus oryzae hexokinase genes in Saccharomyces cerevisiae,” 2010, vol. 277, Accessed: 00, 2020. [Online]. Available: