VECTOR CONSTRUCTION STRATEGIES FOR TRANSFORMATION OF WHEAT PLANT

Date

2013-01-01

Author

BALOĞLU, MEHMET CENGİZ
Battal, Ali
AYDIN, GÜLSÜM
Eroğlu, Ahmet Ümit
Oz, M. T.
KAVAS, MUSA
Öktem, Hüseyin Avni
YÜCEL, MUSTAFA

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

165
views

0
downloads

Gene cloning and vector construction for plant genetic transformation are routine procedures in plant functional genomic studies. The availability of effective transformation vector is one of the pre-requisites for plant transformation studies. Here, we describe the construction of a series of transformation vectors through different genetic engineering techniques for wheat transformation. For this purpose, NAC-type transcription factor genes TaNAC69-1 and TtNAM-B2 were isolated from T. aestivum and T. turgidum, respectively. Then they were cloned into different types of cloning and expression vectors. Besides traditional restriction enzyme digestion and ligation method, Gateway cloning technology which is a fast and reliable alternative cloning method were used for construction of wheat transformation vectors. The transformation vectors constructed in this study are suitable for use in both particle bombardment (biolistic) and Agrobacterium based transformation protocols for wheat plant.

Subject Keywords

Traditional gene cloning, Gatewaygene cloning, Vector construction, Wheat transformation

URI

https://hdl.handle.net/11511/53276

Journal

JOURNAL OF ANIMAL AND PLANT SCIENCES

Collections

Department of Computer Education and Instructional Technology, Article

Suggestions

OpenMETU
Core

CELLULOSE-TRIGGERED SPORULATION IN THE GALACTOSE OXIDASE-PRODUCING FUNGUS CLADOBOTRYUM (DACTYLIUM) DENDROIDES NRRL-2903 AND ITS REIDENTIFICATION AS A SPECIES OF FUSARIUM Ögel, Zümrüt Begüm; BRAYFORD, D; MCPHERSON, MJ (1994-04-01) The production of extracellular galactose oxidase is limited to a few fungal species, including the important plant pathogens Fusarium graminearum and F. moniliforme. The best-studied enzyme is the one produced by the mycoparasitic fungus Cladobotryum (Dactylium) dendroides NRRL 2903. The NRRL 2903 strain was first mis-identified as Polyporus circinatus and later re-determined as Dactylium dendroides, although sporulation was never observed and the fungus was regarded as sterile. Upon growth at 25-degrees-C...
Partially Observable Gene Regulatory Network Control Without a Boundary on Horizon Erdogdu, Utku; Polat, Faruk; Alhajj, Reda (2012-11-09) Gene regulatory networks (GRNs) govern the protein transcription process in the cell and interactions among genes play a vital role in determining the biosynthesis rate of proteins. By using intervention techniques discovered by biological research it is possible to control a GRN, thus promoting or demoting the expression rate of a certain gene. In this work, this control task is studied in a partially observable setting where interventions lack perfect knowledge of the expression level of all genes. Moreov...
Mathematical Modeling and Approximation of Gene Expression Patterns Yılmaz, Fatih; Öktem, Hüseyin Avni (2004-09-03) This study concerns modeling, approximation and inference of gene regulatory dynamics on the basis of gene expression patterns. The dynamical behavior of gene expressions is represented by a system of ordinary differential equations. We introduce a gene-interaction matrix with some nonlinear entries, in particular, quadratic polynomials of the expression levels to keep the system solvable. The model parameters are determined by using optimization. Then, we provide the time-discrete approximation of our time...
Molecular cloning, characterization, and homologous expression of an endochitinase gene from Bacillus thuringiensis serovar morrisoni Okay, Sezer; Özcengiz, Gülay (2011-01-01) The endochitinase gene (chi3023) of Bacillus thuringiensis (Bt) serovar morrisoni strain 3023 was amplified via polymerase chain reaction (PCR) and cloned in Escherichia coli. The ORF of chi3023 (GenBank Accession Number: DQ993175) consists of 2031 nucleotides encoding a 676-residue protein with a calculated molecular mass of 74.5 kDa and a pI value of 6.0. The amino acid sequence of Chi3023 was compared with previously sequenced Bt chitinases and the phylogenetic relationships among them were determined. T...
Integer linear programming based solutions for construction of biological networks Eren Özsoy, Öykü; Can, Tolga; Department of Health Informatics (2014) Inference of gene regulatory or signaling networks from perturbation experiments and gene expression assays is one of the challenging problems in bioinformatics. Recently, the inference problem has been formulated as a reference network editing problem and it has been show that finding the minimum number of edit operations on a reference network in order to comply with perturbation experiments is an NP-complete problem. In this dissertation, we propose linear programming based solutions for reconstruction o...

Citation Formats

M. C. BALOĞLU et al., “VECTOR CONSTRUCTION STRATEGIES FOR TRANSFORMATION OF WHEAT PLANT,” JOURNAL OF ANIMAL AND PLANT SCIENCES, pp. 906–912, 2013, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/53276.