Analysis of self-catalytic maturation capability of galactose oxidase by site-directed mutagenesis

Date

2010-06-01

Author

Kocuklu, B.
Ögel, Zümrüt Begüm
McPherson, M. J.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

44
views

0
downloads

URI

https://hdl.handle.net/11511/53813

Collections

Graduate School of Natural and Applied Sciences, Conference / Seminar

Suggestions

OpenMETU
Core

Analysis of self-processing mechanism of galactose oxidase by site-directed mutagenesis and heterologous expression in Escherichia Coli Gençer, Burçak; Ögel, Zümrüt Begüm; Department of Biotechnology (2005) In this study, self-catalytic maturation of heterologously expressed pro-galactose oxidase was analysed in E.coli by altering some amino acids which were supposed to play a crucial role in pro-peptide removal. Galactose oxidase (GOase; EC 1.1.3.9) from Fusarium graminearum; having a molecular mass of 68kDa, is a monomeric, copper containing enzyme with an unusual thioether bond. The enzyme is produced as a precursor with an additional 8 amino acid pre- and a 17- amino acid pro-sequence at the N terminus. Pr...
Analysis of Methanogenic Community Changes in Anaerobic Digesters with Ozone Pre-treatment Tuncay, Sera; Kucukunsal, Serkan; Kayalı, Osman; İçgen, Bülent (null; 2018-09-10)
Analysis of expression of beta-tubulin isotypes and mutations of class I beta-tubulin gene in docetaxel resistant MCF-7 cells Iseri, Oe. Darcansoy; Kars, M. Demirel; Arpaci, F.; Gündüz, Ufuk (2007-07-01)
Analysis of the genetic determinant for production of the pediocin P of Pediococcus pentosaecus Pep 1 Osmanagaoglu, O; Beyatli, Y; Gündüz, Ufuk; Sacilik, SC (2000-01-01) Pediococcus pentosaceus Pep1 is a vacuum-packaged Turkish sausage isolate which produces a potentially novel bacteriocin of the pediocin (anti-Listeria) family of peptides designated as pediocin P. Curing experiments and plasmid profile analysis indicated that both bacteriocin immunity and production determinants were linked and encoded by 9.0 MDa plasmid, pHD1.0. Attempts to transform purified plasmid pHD1.0 into recipient Escherichia coli JM109 cells by electroporation were successful but none of the E. c...
Analysis of the Carbon Metabolism of Rhodopseudomonas palustris for Biohydrogen Production DOĞAN, EZGİ MELİS; Koku, Harun (2022-06-01)

Citation Formats

B. Kocuklu, Z. B. Ögel, and M. J. McPherson, “Analysis of self-catalytic maturation capability of galactose oxidase by site-directed mutagenesis,” 2010, vol. 277, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/53813.