Polymerase chain reaction vs. conventional culture in detection of bacterial pneumonia agents

Guclu, AU
Baysallar, M
Gözen, Ayşe Gül
Kilic, A
Balkan, A
Doganci, L
Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis are important aetiologies of community-acquired pneumonia (CAP). The rapid laboratory diagnosis of CAP is difficult by conventional culture techniques. Polymerase chain reaction (PCR) is an advanced tool, which may solve this problem. Sputum samples were selected and scored by Gram stain. Conventional identification procedures and PCR analysis were applied to the samples and results were compared statistically. Ranging between the ages of 20-80, 107 patients with suspected bacterial pneumonia admitted to the department of The Chest Diseases of Gulhane Military Medical Academy were included in this study. Sixty-seven of 107 (62.6%) samples were positive in PCR, but only 11 in culture. Streptococcus pneumoniae (40.2%; 43/107) was the most common etiologic agent in PCR. The incidences of H. influenzae and M. catarrhalis were determined as 11.2% (12/107), and 1.9% (2/107), respectively in PCR. Oropharyngeal contamination could be a cause of the false positive results in PCR. If valid sputum were to be obtained, PCR might be more sensitive, reliable and beneficial than culture.


Dede, N. Altay; Acar-Soykut, E.; Gültekin, Güzin Candan (2014-01-01)
Sixty Streptococcus thermophilus and 111 Lactobacillus delbrueckii subsp. bulgaricus isolates from traditionally produced Turkish yogurts were biochemically identified and examined for their technologically important features. Acidification profiles of the isolates were examined. Isolates with acidification rates comparable to those of commercial isolates were also tested for final pHs and acetaldehyde production after 24-h incubation. Selected isolates were also examined for their bacteriophage resistance ...
Poly-beta-hydroxybutyrate production by lactic acid bacteria
Aslim, B; Caliskan, F; Beyatli, Y; Gündüz, Ufuk (1998-02-15)
Poly-beta-hydroxybutyrate was determined in lactic acid bacteria belonging to the genera Lactobacillus, Lactococcus, Pediococcus and Streptococcus. Lactobacilli were grown in MRS broth, the others were grown in Elliker broth medium. Cell biomass was obtained by centrifugation. The cell walls were lysed with sodium hypochlorite. Poly-beta-hydroxybutyrate was extracted using chloroform in a Soxhlet system. Then it was converted to crotonic acid using sulfuric acid and the amount of crotonic acid was measured ...
Enzymatic Mineralization of Hydrogels for Bone Tissue Engineering by Incorporation of Alkaline Phosphatase
Douglas, Timothy E. L.; Messersmith, Philip B.; Chasan, Safak; Mikos, Antonios G.; de Mulder, Eric L. W.; Dickson, Glenn; Schaubroeck, David; Balcaen, Lieve; Vanhaecke, Frank; Dubruel, Peter; Jansen, John A.; Leeuwenburgh, Sander C. G. (Wiley, 2012-08-01)
Alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, is incorporated into three hydrogel biomaterials to induce their mineralization with calcium phosphate (CaP). These are collagen type I, a mussel-protein-inspired adhesive consisting of PEG substituted with catechol groups, cPEG, and the PEG/fumaric acid copolymer OPF. After incubation in Ca-GP solution, FTIR, EDS, SEM, XRD, SAED, ICP-OES, and von Kossa staining confirm CaP formation. The amount of mineral formed decreases in the orde...
Genomic characterization of pseudomonas aeruginosa clinical isolates by pulsed field gel electrophoresis method
Alipour Ghorbani, Nader; Yıldız, Fatih; Durmaz, Rıza; Department of Biotechnology (2014)
Pseudomonas aeruginosa is a common cause of nosocomial infections, particularly in intensive care units (ICUs), bronchoscopy, oncology, and urology. The aim of this study was to characterize P. aeruginosa clinical isolates clonally relatedness by pulsed-field gel electrophoresis (PFGE) typing and it’s cut off value determination. We did retrospective study and analyzed genotypically a collection of 58 clinical isolates recovered during the period 2006–2011 from MESA private hospital microbiology department’...
HACIOGLU, B; Akbulut, Ural (Informa UK Limited, 1991-01-01)
Imidazole was employed as an anionic initiator for the homopolymerization of maleic anhydride in various solvents. Polymerization was very rapid and the obtained products have different colours varying from white to dark brown. The colour of the product was found to depend on the quantity of imidazole added, and the solvent of polymerization. Darkness of polymers are thought to be due to cross-conjugation displaying paramagnetic character. The ESR signals of dark brown polymers were more intense than th...
Citation Formats
A. Guclu, M. Baysallar, A. G. Gözen, A. Kilic, A. Balkan, and L. Doganci, “Polymerase chain reaction vs. conventional culture in detection of bacterial pneumonia agents,” ANNALS OF MICROBIOLOGY, pp. 313–316, 2005, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/53855.