Trehalose polymers for stabilization of industrially important proteins

Date

2016-03-13

Author

Lee, Juneyoung
Ko, Jeong Hoon
Liu, Yang
Lin, En-Wei
Messina, Marco
Bat, Erhan
Nauka, Peter
Wallace, Peter
Ruch, Frank
Maynard, Heather

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URI

https://hdl.handle.net/11511/54442

Journal

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY

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Department of Chemical Engineering, Article

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Trehalose glycopolymer resists allow direct writing of protein patterns by electron-beam lithography Bat, Erhan; Lau, Uland Y.; Maynard, Heather D. (Springer Science and Business Media LLC, 2015-03-01) Direct-write patterning of multiple proteins on surfaces is of tremendous interest for a myriad of applications. Precise arrangement of different proteins at increasingly smaller dimensions is a fundamental challenge to apply the materials in tissue engineering, diagnostics, proteomics and biosensors. Herein, we present a new resist that protects proteins during electron-beam exposure and its application in direct-write patterning of multiple proteins. Polymers with pendant trehalose units are shown to effe...
Trehalose Glycopolymers as Excipients for Protein Stabilization Lee, Juneyoung; Lin, En-Wei; Lau, Uland Y.; Hedrick, James L.; Bat, Erhan; Maynard, Heather D. (American Chemical Society (ACS), 2013-08-01) Herein, the synthesis of four different trehalose glycopolymers and investigation of their ability to stabilize proteins to heat and lyophilization stress are described. The disaccharide, alpha,alpha-trehalose, was modified with a styrenyl acetal, methacrylate acetal, styrenyl ether, or methacrylate moiety resulting in four different monomers. These monomers were then separately polymerized using free radical polymerization with azobisisobutyronitrile (AIBN) as an initiator to synthesize the glycopolymers. ...
Trehalose metabolism in wheat and identification of trehalose metabolizing enzymes under abiotic stress conditions El-Bashiti, Tarek; Yücel, Ayşe Meral; Hamamcı, Haluk; Department of Biotechnology (2003) Trehalose (a-D-glucopyranosyl-1,1-a-D-glucopyranoside) is a non reducing disaccharide of glucose that occurs in a large variety of organisms, ranging from bacteria to invertebrate animals, where it serves as an energy source or stress protectant. Until recently, only few plant species, mainly desiccation tolerant ءresurrection̕ plants, were considered to synthesize trehalose. Although most plant species do not appear to accumulate easily detectable amounts of trehalose, the discovery of genes for trehalose ...
Trehalose, glycogen and ethanol metabolism in the gcr1 mutant of Saccharomyces cerevisiae. Şeker, Tamay (Springer Science and Business Media LLC, 2003-01-01) Since Gcr1p is pivotal in controlling the transcription of glycolytic enzymes and trehalose metabolism seems to be one of the control points of glycolysis, we examined trehalose and glycogen synthesis in response to 2 % glucose pulse during batch growth ingcr1 (glucose regulation-1) mutant lacking fully functional glycolytic pathway and in the wild-type strain. An increase in both trehalose and glycogen stores was observed 1 and 2 h after the pulse followed by a steady decrease in both the wild-type and the...
Trehalose and glycogen metabolism of gcr1 mutants of saccharomyces cerevisiae Şeker, Tamay; Hamamcı, Haluk; Türker, Mustafa; Department of Biotechnology (2002) S. cerevisiae is one of the most important microorganisms with respect to its long history and being model organism. Trehalose and glycogen are the major carbohydrate stores in yeast cell and they are typically hall-marks of rapid adaptations of yeast cells to environmental changing conditions such that trehalose has a significant role in preservation and survival of the yeast cells under adverse conditions, while glycogen is the main energy source. Trehalose metabolism has also a regulatory role in glucose...

Citation Formats

J. Lee et al., “Trehalose polymers for stabilization of industrially important proteins,” ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, pp. 0–0, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/54442.