GLUCOSE-OXIDASE IMMOBILIZED ON GELATIN BY VARIOUS CROSS-LINKERS

Date

1993-01-01

Author

ELCIN, YM
SUNGUR, S
Akbulut, Ural

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

29
views

0
downloads

Immobilization of glucose oxidase have been realized with photographic gelatin as support for chemical coupling. Chromium acetate, potassium chromium sulfate and formaldehyde were used as crosslinking agents to immobilize glucose oxidase on film strips. Enzyme, crosslinker concentrations, temperature, pH and reuse number on native and immobilized glucose oxidase activity and water contents of unused and used films were investigated. The best results were achieved with the crosslinker chromium acetate as 50% maximum activity after 40 assays.

URI

https://hdl.handle.net/11511/54562

Journal

JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

POLYESTER FILM STRIPS COATED WITH PHOTOGRAPHIC GELATIN CONTAINING IMMOBILIZED GLUCOSE-OXIDASE HARDENED BY CHROMIUM(III) SULFATE ELCIN, YM; Akbulut, Ural (Elsevier BV, 1992-01-01) Glucose oxidase was immobilized into photographic gelatin hardened by chromium(III) sulphate. The enzyme-gelatin mixture was coated on polyester film strips which allowed easy and simple handling during assays. The effect of gelatin and cross-linker concentrations on water content and enzymatic activity was studied. The effect of pH during immobilization and that of incubation temperature on maximum activity were examined- Enzyme leakage tests were carried out during reuse number studies. Consecutive use of...
Immobilization of glucose oxidase onto gelatin for biosensor construction Emregul, E; Sungur, S; Akbulut, Ural (Informa UK Limited, 2005-01-01) The properties of a glucose biosensor made by immobilization of glucose oxidase onto gelatin in a layer of electrochemically deposited polyaniline have been investigated. Glucose oxidase was immobilized within gelatin cross-links with chromium(III) acetate. The glucose oxidase biosensor was developed by forming a polyaniline-deposited electrode surface as support for the immobilized enzyme gel, in order to increase its durability. The polyaniline/gelatin/glucose oxidase biosensor has been characterized usin...
Immobilization of glucose isomerase in surface-modified alginate gel beads TÜMTÜRK, HAYRETTİN; DEMİREL, Gökhan; ALTINOK, HAYDAR; AKSOY, SERPİL; Hasırcı, Nesrin (2008-04-01) In this study, glucose isomerase enzyme was entrapped into modified and nonmodified calcium alginate gel beads. Various characteristics of free and immobilized enzymes such as the optimum pH, temperature and dependence of activity on storage and operational stability were evaluated. The optimum pH and temperature of free and immobilized glucose isomerase were found to be the same values as 7.5 and 60C, respectively. For free and immobilized enzymes, kinetic parameters were calculated as 1.79 x 10(-2) and 8....
Synthesis of calcium carbonate particles for biomedical applications Oral, Çağatay Mert; Ercan, Batur; Department of Metallurgical and Materials Engineering (2020) Calcium carbonate (CaCO3) particles have been widely used in biomedical applications owing to their biocompatibility and biodegradability. In order to effectively utilize CaCO3 particles in biomedical applications, their physical and chemical properties should be systematically controlled. However, this is a challenging task due to the presence of three different anhydrous CaCO3 polymorphs having complex crystallization behavior. In this thesis, CaCO3 particles were synthesized at distinct environments to c...
Poly(hydroxybutyrate-co-hydroxyvalerate) nanocapsules as enzyme carriers for cancer therapy: an in vitro study Baran, ET; Ozer, N; Hasırcı, Vasıf Nejat (2002-05-01) In the present paper, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nanocapsules were prepared by a double emulsion-solvent evaporation procedure (w/o/w) for the encapsulation of model enzymes (L-asparaginase, catalase, glucose oxidase) and bovine serum albumin. To increase the encapsulation efficiency and activity of the encapsulated enzyme, numerous modifications were made in the compositions of the phases of double emulsion. For the preparation of low molecular weight PHBV, the polymer was treated with so...

Citation Formats

Y. ELCIN, S. SUNGUR, and U. Akbulut, “GLUCOSE-OXIDASE IMMOBILIZED ON GELATIN BY VARIOUS CROSS-LINKERS,” JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY, pp. 137–147, 1993, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/54562.