Effect of modification of sheep lung cytochrome P450LgM2(2B) by ethylacetimidate in hydroxylation activity

Date

1998-11-01

Author

Adalı, Orhan

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

75
views

0
downloads

Cytochrome P450LgM2(2B) purified from sheep lung microsomes was treated chemically using ethylacetimidate at pH 8.5 for 2 hours at room temperature. Ethylacetimidate modification of essential lysine residues of lung P450LgM2 caused an inhibition of catalytic activity P450 towards substrate benzphetamine. Benzphetamine N-demethylase activity of the reconstituted system containing modified P450LgM2 and purified sheep lung NADPH-cytochrome P450 reductase was inhibited by 93%. This inhibition may be due to an impaired interaction of P450LgM2 and reductase via electrostatic farces.

Subject Keywords

Cytochrome P450LgM2(2B), Sheep lung, Benzphetamine, Ethylacetimidate

URI

https://hdl.handle.net/11511/54679

Journal

BIOCHEMICAL ARCHIVES

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Influence of pH conditions on metabolic regulations in serine alkaline protease production by Bacillus licheniformis Çalık, Pınar; Calik, G; Ozdamar, TH (2002-10-03) The effects of the controlled and uncontrolled pH conditions, as well as of the value of initial pH in the range of 7-7.5, on serine alkaline protease (SAP) production by Bacillus licheniformis were investigated on a defined medium with the single carbon source glucose in batch bioreactors. Besides uncontrolled pH operations, growth phase, production phase, and entire process control strategies were also applied. The concentrations of the product (SAP) and by-products, i.e. neutral protease, amylase, amino ...
Effect of high hydrostatic pressure on the profile of proteins extracted from Betula pendula pollens Altuner, Ergin Murat; Ceter, Talip; Alpas, Hami (2014-10-02) High hydrostatic pressure (HHP) has high success potential in pollen protein extraction, but its effect on pollen protein profiles has not been studied yet. The aim of this study is to put forward whether HHP processing causes a change in the protein profiles extracted from pollens or not. In this study, proteins extracted from Betula pendula pollens were studied at 100, 200 and 300 MPa at room temperature for 5 min. In addition, the efficiency of three different extraction solvents, namely phosphate buffer...
Effects of nickel, chromium and initial feed concentration on the batch growth of a microbial consortium developed from sewage Gökçay, CELAL FERDİ; Dilek, Filiz Bengü (Informa UK Limited, 1991-01-01) Effects of nickel, chromium and substrate concentration on the microbial growth of acclimatized and unacclimatized microbes of sewage origin were studied in batch cultures at a constant temperature of 25 °C, at pH 7.0. A synthetic medium supplemented with Ni(II) (5 and 10 mgl‐1) or Cr(VI) (1 and 50 mgl‐1) or with their combinations and at various feed concentrations (650, 975, 1300 mgl‐1) was employed to investigate the effects on the specific growth rate (μm) of the microbial culture. The experimental resu...
Effect of pH on erythropoietin production by recombinant Pichia pastoris in fed-batch operation Soyaslan, Elif Şükran; Çalık, Pınar; Department of Chemical Engineering (2010) In this study, the effects of pH on therapeutically important protein, recombinant human erythropoietin (rhuEPO), production by Pichia pastoris was investigated at pH=4.0, 4.5, 5.0, 5.5 and 6.0. rHuEPO production was started by methanol induction in fed-batch mode. The highest cell concentration was obtained at pH=4.5 as 81.4 g L-1. The co-substrate substrate sorbitol, which was added batch-wise, was consumed at t=15 h of the operations at pH=4.0, 4.5 and 5.0. However as the pH increases above pH=5.0 the so...
EFFECT OF THE LACTOPEROXIDASE SYSTEM ON THE ACTIVITY OF STARTER CULTURES FOR YOGURT PRODUCTION Basaga, H.; Dik, Tunay (VOLKSWIRTSCHAFTLICHER VERLAG, 1994) The LP system in raw milk was activated by the addition of equimolar concentrations (0.25 mM) of hydrogen peroxide (H2O2) and thiocyanate (SCN) as KSCN and utilizing the inherent milk lactoperoxidase (LP). H2O2 and SCN- concentrations were determined during the experiments by using spectrophotometric methods and found to decrease to 1.5 mug/ml and 0.4 mug/ml respectively in 5 h. A relatively small amount of H2O2 was produced in the untreated milk which then was found to decrease. The activated sample, at th...

Citation Formats

O. Adalı, “Effect of modification of sheep lung cytochrome P450LgM2(2B) by ethylacetimidate in hydroxylation activity,” BIOCHEMICAL ARCHIVES, pp. 241–246, 1998, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/54679.