Enzyme kinetics of flavin-dependent monooxygenases modulate the observable isotope fractionation during monooxidation of 4-nitrophenol

Wijker, Reto S.
Kurt, Zöhre
Spain, Jim C.
Bolotin, Jakov
Hofstetter, Thomas B.


Enzyme entrapment in polymer coated liposomes
Özden, M. Yaşar; Hasırcı, Vasıf Nejat; Department of Biology (1990)
Enzyme immobilization to modified and patterned surfaces for biocatalytically active surface preparation
Cinar, M.; Eker, Z.; Karakaş, Gürkan; Bakir, U. (Elsevier BV, 2009-09-01)
Enzyme catalyzed hydroxymethylation of aromatic aldehydes with formaldehyde. Synthesis of hydroxyacetophenones and (S)-benzoins
Demir, Ayhan Sıtkı; Ayhan, P; Igdir, AC; Duygu, AN (Elsevier BV, 2004-07-26)
Benzaldehyde lyase from the Pseudomonas Fluorescens catalyzed reaction of aromatic aldehydes with formaldehyde providing 2-hydroxy-1-arylethan-1-one in high yields via an acyloin linkage. Kinetic resolution of rac-benzoins with formaldehyde providing (S)-benzoins and 2-hydroxy-1-arylethan-1-one via C-C bond cleavage and a bond formation reaction.
Enzyme-Catalyzed Stereoselective Synthesis of Two Novel Carbasugar Derivatives
Gumus, Aysegul; Tanyeli, Cihangir (2010-01-01)
Enzymatic resolution of racemic 1,4,5,6-tetrachloro-2-(hydroxymethyl)-7,7-dimethoxybicyclo[2.2.1]hept-5-ene (rac-1) using various lipases in vinyl acetate as acetyl source was studied. The obtained enantiomerically enriched (+)-(1,4,5,6-tetrachloro-7,7-dimethoxybicyclo[2.2.1]hept-5-en-2-yl)methyl acetate ((+)-2; 94% ee), upon treatment with Na in liquid NH3, followed by Amberlyst-15 resin in acetone, provided (-)-5-(hydroxymethyl)bicyclo[2.2.1]hept-2-en-7-one ((-)-7), which is a valuable precursor for the s...
Enzyme Catalyzed Trans-Benzoin Condensation
BİLİR, Gökçil; AYHAN SITKI, Demir; Özçubukçu, Salih (2018-04-01)
Benzaldehyde lyase (BAL) is an enzyme that is used in the C-C bond cleavage and formation which was isolated first from Pseudomonas fluorescens Biovar I. It requires thiamine diphosphate (ThDP) and Mg(II) ions as cofactors. In this work, BAL was used as an enzymatic catalysis for the trans-benzoin condensation reaction between racemic benzoins and benzyloxyacetaldehyde to form unsymmetrical benzoin products with moderate enantiomeric excesses. (S)-benzoin derivatives remained unreacted at the end of the rea...
Citation Formats
R. S. Wijker, Z. Kurt, J. C. Spain, J. Bolotin, and T. B. Hofstetter, “Enzyme kinetics of flavin-dependent monooxygenases modulate the observable isotope fractionation during monooxidation of 4-nitrophenol,” 2011, vol. 242, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/55306.