Characterization of beta-galactosidase immobilized into poly(hydroxyethylmethacrylate) membranes

Date

1998-05-01

Author

Adalı, Orhan
Arica, MY

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

136
views

0
downloads

beta-galactosidase was immobilized into pHEMA membranes with the highest specific activity yield of 9.5%. The specific activity of the entrapped enzyme was found to be decreased as the enzyme loading increased in pHEMA membranes. The optimum pH and temperature for maximum activity of the immobilized beta-galactosidase was found to be at pH 7.5 and 50 degrees C, respectively, and were the same as native enzyme. K-m and V-max values for the free enzyme were found to be 0.256 mM and 26.6 mu mole/min/mg, respectively. K-m value of immobilized beta-galactosidase was found to be increased about 3 folds upon immobilization. Operational, thermal and storage stability of beta-galactosidase were found to increase with immobilization. Immobilized enzyme preparation was reused in 15 cycles without significant loss in activity.

Subject Keywords

Beta-galactosidase, Immobilization

URI

https://hdl.handle.net/11511/56039

Journal

BIOCHEMICAL ARCHIVES

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Characterization of β-galactosidase immobilized into poly(hydroxyethyl methacrylate) membranes Adalı, Orhan; Arica, M.y. (1998-01-01) β-galactosidase was immobilized into pHEMA membranes with the highest specific activity yield of 9.5%. The specific activity of the entrapped enzyme was found to be decreased as the enzyme loading increased in pHEMA membranes. The optimum pH and temperature for maximum activity of the immobilized βgalactosidase was found to be at pH 7.5 and 50°C, respectively, and were the same as native enzyme. K(m) and V(max) values for the free enzyme were found to be 0.256 mM and 26.6 μmole/min/mg, respectively. K(m) va...
Comparison of beta-galactosidase immobilization by entrapment in and adsorption on poly(2-hydroxyethylmethacrylate) membranes Baran, T; Arica, MY; Denizli, A; Hasırcı, Vasıf Nejat (1997-12-01) beta-Galactosidase was immobilized in/on poly(2-hydroxyethyl methacrylate) (pHEMA) membranes by two different methods: adsorption on Cibacron F3GA derivatized pHEMA membranes (pHEMA-CB), and entrapment in the bulk of the pHEMA membranes. The maximum beta-galactosidase adsorption on pHEMA-CB membranes was obtained as 95.6 mu g cm(-2) in 2.0 mg cm(-3) enzyme solution. The adsorption phenomena appeared to follow a typical Langmuir isotherm. In the entrapment, an increase in beta-galactosidase loading resulted ...
Preparation of Chitosan-Coated Magnetite Nanoparticles and Application for Immobilization of Laccase Kalkan, Nuzhet Ayca; AKSOY, SERPİL; Aksoy, Eda Ayse; Hasırcı, Nesrin (2012-01-15) In this study, immobilization of laccase (L) enzyme on magnetite (Fe(3)O(4)) nanoparticles was achieved, so that the immobilized enzyme could be used repeatedly. For this purpose, Fe(3)O(4) nanoparticles were coated and functionalized with chitosan (CS) and laccase from Trametes versicolor was immobilized onto chitosan-coated magnetic nanoparticles (Fe(3)O(4)-CS) by adsorption or covalent binding after activating the hydroxyl groups of chitosan with carbodiimide (EDAC) or cyanuric chloride (CC). For chitosa...
Characterization of defect states in Ga-rich gallium arsenide crystals by thermally stimulated current YILDIRIM, TACETTİN; Hasanlı, Nızamı; TÜZEMEN, Sebahattin (2018-06-01) The trap levels in Ga-rich GaAs crystals were studied in the temperature range of 10-300 K using thermally stimulated currents (TSC) technique. During the experiments we utilized a constant heating rate of 0.2 K/s. Experimental evidence is found for two electron trapping centers in the crystal with activation energies of 44 and 50 meV. The analysis of the experimental TSC curve suggests slow retrapping. The capture cross sections of the traps were determined as 8.8 x 10(-25) and 1.0 x 10(-25) cm(2) with con...
Characterization of trap centers in Gd2O3 nanoparticles by low temperature thermoluminescence measurements Delice, S.; IŞIK, MEHMET; Hasanlı, Nızamı (2018-01-01) Trapping centers in Gd2O3 nanoparticles were investigated using thermoluminescence (TL) measurements in the below room temperature region of 10-280 K. Seven peaks having peak maximum temperatures between 30 and 252 K were observed in the TL spectra measured at constant heating rate of 0.3 K/s. Activation energies, order of kinetics and frequency factors were reported using three different analysis techniques: curve fitting, initial rise and peak shape methods. Activation energies of the trapping centers wer...

Citation Formats

O. Adalı and M. Arica, “Characterization of beta-galactosidase immobilized into poly(hydroxyethylmethacrylate) membranes,” BIOCHEMICAL ARCHIVES, pp. 123–129, 1998, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/56039.