Molecular characterization of zeta class glutathione S-transferases from Pinus brutia Ten.

Date

2015-09-01

Author

ÖZTETİK, ELİF
KÖÇKAR, FERAY
ALPER, MELTEM
İşcan, Mesude

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Glutathione transferases (GSTs; EC 2.5.1.18) play important roles in stress tolerance and metabolic detoxification in plants. In higher plants, studies on GSTs have focussed largely on agricultural plants. There is restricted information about molecular characterization of GSTs in gymnosperms. To date, only tau class GST enzymes have been characterized from some pinus species. For the first time, the present study reports cloning and molecular characterization of two zeta class GST genes, namely PbGSTZ1 and PbGSTZ2 from Pinus brutia Ten., which is an economically important pine native to the eastern Mediterranean region and have to cope with several environmental stress conditions. The PbGSTZ1 gene was isolated from cDNA, whereas PbGSTZ2 was isolated from genomic DNA. Sequence analysis of PbGSTZ1 and PbGSTZ2 revealed the presence of an open reading frame of 226 amino acids with typical consensus sequences of the zeta class plant GSTs. Protein and secondary structure prediction analysis of two zeta class PbGSTZs have shared common features of other plant zeta class GSTs. Genomic clone, PbGSTZ2 gene, is unexpectedly intronless. Extensive sequence analysis of PbGSTZ2, with cDNA clone, PbGSTZ1, revealed 87% identity at nucleotide and 81% identity at amino acid levels with 41 amino acids differences suggesting that genomic PbGSTZ2 gene might be an allelic or a paralogue version of PbGSTZ1.

Subject Keywords

Isozymes , Glutathione Transferases , Gymnosperms , Glutathione Transferases Zeta

URI

https://hdl.handle.net/11511/56692

Journal

JOURNAL OF GENETICS

DOI

https://doi.org/10.1007/s12041-015-0538-5

Collections

Department of Biology, Article

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Glutathione S-Transferase activities and glutathione levels in needles of drought stressed Pinus Brutia Ten. trees Akyüz Yılmaz, Cansu (2014-01-01) Aim: Glutathione S-transferases (GST) take roles under stress conditions by the conjugation of Glutathione (GSH) to electrophilic substrates to increase their solubility and facilitating further metabolic processing. This is one of the main pathways functioning in stress resistance and, for dought stress, there is not yet a study on Pinus brutia Ten. which is a very important forest tree of Turkey.
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Citation Formats

E. ÖZTETİK, F. KÖÇKAR, M. ALPER, and M. İşcan, “Molecular characterization of zeta class glutathione S-transferases from Pinus brutia Ten.,” JOURNAL OF GENETICS, pp. 417–423, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/56692.