Growth hormone (GH), prolactin (PRL), and diacylglycerol acyltransferase (DGAT1) gene polymorphisms in Turkish native cattle breeds

Kepenek, Eda Seyma
Dinc, Havva
Ozer, Fusun
Sonmez, Gizem
Togan, İnci Zehra
SOYSAL, Mehmet Ihsan
The aim of this study was to determine the genetic diversity of 4 native Turkish cattle breeds, based on the growth hormone (GH), prolactin (PRL), and diacylglycerol acyltransferase (DGAT1) genes. In order to study the polymorphisms in these genes, the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) method was performed. A 329-bp fragment and a 223-bp fragment of GH, a 156-bp fragment of PRL, and a 411-bp fragment of DGAT1, thus 4 loci of 3 genes, were amplified via PCR. These fragments were then restricted with the enzymes MspI, Alu1, RsaI, and CfrI, respectively. In this study, 2 types of alleles, (+) and (-) for the GH-MspI, L and V for the GH-Alu1, A and B for the PRL, and K and A for the DGAT1 loci, were observed. The results of the present study will contribute to the polymorphism data on the world's cattle breeds. Furthermore, the above-mentioned allele frequencies of Turkish native breeds are evaluated in relation to their genetic relatedness, and to infer their milk production properties on the basis of the available literature. In turn, these results can be utilized for future breeding programs of dairy cattle in Turkey.


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In this study the genetic diversity in Turkish native sheep breeds was investigated based on microsatellite DNA loci. In total, 423 samples from 11 native and crossbreed Turkish sheep breeds (Akkaraman, Morkaraman, Kivircik, Ivesi, Dagliç, Karayaka, Hemsin, Norduz, Kangal, Konya Merinosu, Türkgeldi) and one Iraqi breed (Hamdani) were analyzed by sampling from breeding farms and local breeders. After excluding close relatives by Kinship analysis, the genetic variation within breeds was estimated as gene dive...
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The present study was conducted to confirm the recently suggested taxonomic status of gazelles in Turkey and also to investigate genetic diversity that exists between and within populations of Gazella marica (One from Kızılkuyu State Farm, Şanlıurfa, n=48; one from Erikçe State Farm, Gaziantep, n=25) and Gazella gazella (population from Kırıkhan County, Hatay, n=4). In the frame of the study, partial mtDNA cyt-b sequence (amplified by the primers: L14724, H15149), 17 microsatellite loci (RT1, ETH10, OARFCB3...
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The objective of this study was to assess the genetic diversity and genetic relationships among 59 Turkish barley (Hordeum vulgare L.) germplasm lines (maintained for scald disease resistance breeding) using sequence-related amplified polymorphism (SRAP) markers. Seventeen SRAP primer combinations produced 83 polymorphic markers, with a mean polymorphism of 73.5%. The dendrogram created based on Nei's unweighted-pair group method using arithmetic average (UPGMA) indicated that there were 4 main clusters, wh...
Polymorphism of Prolactin (PRL), Diacylglycerol Acyltransferase (DGAT-1) and Bovine Solute Carrier Family 35 Member 3 (SLC35A3) genes in native cattle breeds and its implication for Turkish cattle breeding
Kepenek, Eda Şeyma; Togan, İnci Zehra; Department of Biology (2007)
In the present study samples from four native Turkish Cattle Breeds; South Anatolian Red (n= 48), East Anatolian Red (n= 34), Anatolian Black (n= 42) and Turkish Grey (n=46) and elite bulls of Holstein (n=21) were genotyped with respect to two milk production enhancer genes, Prolactin (PRL) and Diacylglycerol acyltransferase (DGAT1), and one disease (Complex Vertebral Malformation) causing gene (SLC35A3). A allele frequency for PRL gene, believed to be positively associated with the milk yield in cattle, ra...
Citation Formats
E. Ö. ÜNAL et al., “Growth hormone (GH), prolactin (PRL), and diacylglycerol acyltransferase (DGAT1) gene polymorphisms in Turkish native cattle breeds,” TURKISH JOURNAL OF ZOOLOGY, pp. 734–748, 2015, Accessed: 00, 2020. [Online]. Available: