Isothermal microcalorimetric study of the pH dependence of the interactions between a cellulase and a beta-blocker

Date

2004-08-01

Author

Gotmar, G
Özen, Can
Serpersu, E
Guiochon, G

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

25
views

0
downloads

The influence of the pH on the complexation equilibria between (S)- or (R)-alprenolol and the cellulase Cel7A was investigated by isothermal titration calorimetry. The results obtained agree with those of previous, similar studies of the same equilibria in which the protein was immobilized on silica particles, packed in a chromatographic column. The association constant and the complexation enthalpy and entropy of the (S)-enantiomer increase with increasing pH. For (R)-alprenolol, the binding is endothermic at all pH values. Thus, for both enantiomers in the pH range 5.5-6.8, the binding is an entropically driven process.

Subject Keywords

Organic Chemistry, Analytical Chemistry, Biochemistry, General Medicine

URI

https://hdl.handle.net/11511/57795

Journal

JOURNAL OF CHROMATOGRAPHY A

DOI

https://doi.org/10.1016/j.chroma.2004.06.089

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Cettaertalyst-Free Hydrogenation of Alkenes and Alkynes with Hydrazine in the Presence of Oxygen Menges, Nurettin; Balcı, Metin (Georg Thieme Verlag KG, 2014-03-17) A series of alkenes and alkynes was subjected to reduction with hydrazine hydrate in ethanol in the presence of oxygen. An efficient method was developed for the reduction of C-C double bonds and C-C triple bonds with diimide, generated in situ from hydrazine hydrate by oxidation with oxygen. The reduction process proceeded for 24-48 hours with high chemoselectivity and excellent yields. This reduction procedure offers synthetic advantages over metal-catalyzed hydrogenation as well as other systems.
Electrochemical and quantum chemical studies on mitomycin and adriamycin Ozalp-Yaman, S; Önal, Ahmet Muhtar; Turker, L (Elsevier BV, 2003-06-25) In-situ spectroelectrochemical redox behaviour of two prominent chemotherapeutic agents, mitomycin and adriamycin were studied at constant potential. AM 1 (UHF) type quantum chemical calculations on the neutral as well as radical anion and cation forms of mitomycin and adriamycin were performed.
Transition structures, energetics, and nucleus-independent chemical shifts for divinylcyclobutene-to-cyclooctatriene rearrangement: A DFT study Zora, Metin (American Chemical Society (ACS), 2004-02-06) The minimum energy reaction paths and nucleus-independent chemical shifts (NICS) for the Cope rearrangement of cis-3,4-divinylcyclobutene, obtained by (U)B3LYP/6-31G* calculations, are reported. Three transition structures (endo-boatlike, chairlike, and exo-boatlike) have been located, giving rise to formation of cis,cis,cis-, cis,cis,trans-, and trans,cis, trans- 1, 3,5-cyclooctatrienes, respectively. The minimum energy path proceeds through an endo-boatlike, aromatic transition structure. The reaction pat...
Voltammetric methods of reboxetine analysis and the mechanism of its electrode reactions Altunöz Erdoğan, Deniz; Erk, Nevin; Kilic, Esma (Walter de Gruyter GmbH, 2013-05-01) Reboxetine (RBX) electrochemical redox behavior at hanging mercury drop (HMDE) and glassy carbon electrodes (GCE) was studied in various pH Britton-Robinson universal buffers using cyclic voltammetry and square-wave voltammetry. RBX was reduced at the HMDE and oxidized at the GCE with reversible adsorption controlled and irreversible diffusion controlled processes respectively. The anodic peak is due to the amine and the cathodic peak may correspond to oxygen protonation. An oxidation reaction mechanism is ...
High temperature bromination. Part 18: Bromination of benzonorbornadiene derivatives: Polybrominated benzonorbornenes and benzonorbornadienes Dastan, A; Balcı, Metin (Elsevier BV, 2005-06-06) The low and high temperature bromination reactions of bromobenzonorbornadiene derivatives were studied and the possible role of a neighboring group in rearrangements was investigated. New polybrominated benzonorbornadiene and benzonorbornene derivatives were synthesized. All compounds were characterized property Using NMR spectroscopy.

Citation Formats

G. Gotmar, C. Özen, E. Serpersu, and G. Guiochon, “Isothermal microcalorimetric study of the pH dependence of the interactions between a cellulase and a beta-blocker,” JOURNAL OF CHROMATOGRAPHY A, pp. 49–53, 2004, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/57795.