Isothermal microcalorimetric study of the pH dependence of the interactions between a cellulase and a beta-blocker

Date

2004-08-01

Author

Gotmar, G
Özen, Can
Serpersu, E
Guiochon, G

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The influence of the pH on the complexation equilibria between (S)- or (R)-alprenolol and the cellulase Cel7A was investigated by isothermal titration calorimetry. The results obtained agree with those of previous, similar studies of the same equilibria in which the protein was immobilized on silica particles, packed in a chromatographic column. The association constant and the complexation enthalpy and entropy of the (S)-enantiomer increase with increasing pH. For (R)-alprenolol, the binding is endothermic at all pH values. Thus, for both enantiomers in the pH range 5.5-6.8, the binding is an entropically driven process.

Subject Keywords

Organic Chemistry, Analytical Chemistry, Biochemistry, General Medicine

URI

https://hdl.handle.net/11511/57795

Journal

JOURNAL OF CHROMATOGRAPHY A

DOI

https://doi.org/10.1016/j.chroma.2004.06.089

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

G. Gotmar, C. Özen, E. Serpersu, and G. Guiochon, “Isothermal microcalorimetric study of the pH dependence of the interactions between a cellulase and a beta-blocker,” JOURNAL OF CHROMATOGRAPHY A, pp. 49–53, 2004, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/57795.