Immobilization of a-amylase into photographic gelatin by chemical cross-linking

Download
012887.pdf
1990
Bayramoğlu, Zübeyde
Show full item record
77
views
0
downloads
https://hdl.handle.net/11511/6131
Graduate School of Natural and Applied Sciences, Thesis

Suggestions

IMMOBILIZATION OF ALPHA-AMYLASE INTO PHOTOGRAPHIC GELATIN BY CHEMICAL CROSS-LINKING
BAYRAMOGLU, Z; Akbulut, Ural; SUNGUR, S (Elsevier BV, 1992-01-01)
Alpha-amylase was immobilized into photographic gelatin by chemical cross-linking with chromium (III) acetate and chromium (III) sulphate. Cellulose triacetate film strips, enabled simple handling when coated with an alpha-amylase-gelatin mixture, accomplishing a high degree of durability during consecutive immersions into reaction media. The optimum conditions for pH, substrate concentration, temperature, incubation time and storing conditions of free and immobilized alpha-amylase were determined. The effe...
Immobilization of invertase in conducting polypyrrole polytetrahydrofuran graft polymer matrices
Kizilyar, N; Akbulut, Ural; Toppare, Levent Kamil; Ozden, MY; Yagci, Y (1999-06-24)
Four different polypyrrole/polytetrahydrofuran/invertase electrodes were constructed by the entrapment of invertase in conducting polymer matrices via electropolymerization. Immobilization of the enzyme was achieved by the application of a 1.1 V constant potential to a platinum electrode for 30 min in a solution containing 0.01 M pyrrole, 0.4 mg/ml invertase and 0.4 mg/ml sodium dodecyl sulphate. Performance of each electrode was optimized by examining the effects of pH and temperature on the properties of ...
Immobilization of invertase in conducting polypyrrole/PMMA-co-PMTM graft copolymers
Yildiz, HB; Kiralp, S; Toppare, Levent Kamil; Yagci, Y (Wiley, 2005-04-15)
In this study, invertase was immobilized in copolymer electrodes constructed. Three different types of polymethyl methacrylate-co-polymethyl thienyl methacrylate matrices were used to obtain copolymers that were characterized by FT-IR spectroscopy. Immobilization of enzymes was carried out by the entrapment of the enzyme in conducting polymer matrices during electrochemical polymerization of pyrrole through thiophene moieties of polymers. Immobilization of the enzyme was achieved by application of 1.0 V con...
IMMOBILIZATION OF BETA-GALACTOSIDASE ONTO GELATIN BY GLUTARALDEHYDE AND CHROMIUM(III) ACETATE
SUNGUR, S; Akbulut, Ural (Wiley, 1994-03-01)
Beta-Galactosidase (EC 3.2.1.23) was immobilised onto a gelatin carrier system by crosslinking. Glutaraldehyde and chromium(III) acetate were used as crosslinking agents. The effect of pH on enzyme activity, kinetic parameters and reusability of the immobilised enzyme were investigated. To obtain a better relative activity, a method was developed and further modified to increase the surface area of the support system by using polyacrylamide. Relative activity enhancement of 68% was achieved in the gelatin-g...
Immobilization of catalase in poly(isopropylacrylamide-co-hydroxyethylmethacrylate) thermally reversible hydrogels
Arica, MY; Öktem, Hüseyin Avni; Oktem, Z; Tuncel, SA (1999-09-01)
Catalase was entrapped in thermally reversible poly (isopropylacrylamide-co-hydroxyethyl-methacrylate) (pNIPAM/HEMA) copolymer hydrogels. The thermoresponsive hydrogels, in cylindIl-rical geometry, were prepared in an aqueous buffer by redox polymerization. It was observed that upon entrapment, the activity retention of catalase was decreased between 47 and 14%, and that increasing the catalase loading of hydrogel adversely affected the activity. The kinetic behaviour of the entrapped enzyme was investigate...
Citation Formats
Z. Bayramoğlu, “Immobilization of a-amylase into photographic gelatin by chemical cross-linking,” Middle East Technical University, 1990.
METU IIS - Integrated Information Service open@metu.edu.tr