In vitro susceptibilities of Candida spp. to panomycocin, a novel exo-beta-1,3-glucanase isolated from Pichia anomala NCYC 434

Date

2007-01-01

Author

Izgue, Fatih
Altinbay, Demet
Tuereli, Akif Emre

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Panomycocin, the killer toxin of Pichia anomala NCYC 434 (K5), is a 49 kDa monomeric glycoprotein with exo-beta-1,3-glucanase activity (patent pending). In this study we evaluated the in vitro activity of panomycocin against a panel of 109 human isolates of seven different pathogenic Candida spp. using microdilution and time-kill methods. Panomycocin was most active against C. tropicalis, C. pseudotropicalis and C. glabrata with MIC90 values of 1 mu g/ml. It displayed significant activity against C. albicans and C. parapsilosis with MIC90 values of 4 and 2 mu g/ml, respectively. For C. krusei, the MIC90 value was 8 mu g/ml. Panomycocin was fungicidal against all the tested Candida spp. The MFC values were only one or 2 dilutions higher than the MICs with the exception of C. krusei isolates with MFCs greater than or equal to 4 X MIC. Results of this study indicated that panomycocin could be considered as a natural antifungal agent against Candida infections and has significant potential for further investigation.

Subject Keywords

Immunology, Microbiology, Virology, Yeast killer toxin, Exo-beta-1,3-glucanase, Susceptibility testing, Candida species

URI

https://hdl.handle.net/11511/66753

Journal

MICROBIOLOGY AND IMMUNOLOGY

DOI

https://doi.org/10.1111/j.1348-0421.2007.tb03975.x

Collections

Department of Biology, Article

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Citation Formats

F. Izgue, D. Altinbay, and A. E. Tuereli, “In vitro susceptibilities of Candida spp. to panomycocin, a novel exo-beta-1,3-glucanase isolated from Pichia anomala NCYC 434,” MICROBIOLOGY AND IMMUNOLOGY, pp. 797–803, 2007, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/66753.