In vitro activity of caspofungin compared to amphotericin B, fluconazole, and itraconazole against Candida strains isolated in a Turkish University Hospital

Arikan, S
Sancak, B
Hascelik, G
We investigated the in vitro activity of caspofungin compared to amphotericin B, fluconazole, and itraconazole against clinical strains of Candida spp. ( n = 239). Antifungal susceptibility tests were done in accordance with NCCLS M27-A2 microdilution method and the results were read after 24 and 48 h. In general, 24 h MIC readings were similar to those at 48 h for most isolates and all antifungal agents. Caspofungin was active against all species tested. Caspofungin MICs of Candida parapsilosis were slightly higher than those for other Candida spp. Caspofungin MIC (mu g/ml) ranges at 24 h for C. albicans, C. glabrata, C. tropicalis, C. parapsilosis, C. kefyr, C. krusei, C. lusitaniae, C. norvegensis, C. guilliermondii and C. lipolytica were 0.06 - 2, 0.125 - 2, 0.125 - 2, 1 - 4, 0.125 - 2, 1 - 2, 0.5 - 2, 0.5 - 1, 0.5 - 2 and 1 - 2, respectively. Eagle ( paradoxical) effect was observed in 31 and 8% of the isolates at highest concentrations of caspofungin and itraconazole, respectively. The activity of caspofungin against fluconazole- and/or itraconazole-resistant isolates was similar to that detected for the susceptible ones. We conclude that caspofungin appears as a promising antifungal agent with enhanced activity against Candida, including the azole-resistant strains.


In vitro activity of panomycocin, a novel exo-beta-1,3-glucanase isolated from Pichia anomala NCYC 434, against dermatophytes
Izgu, Fatih; Altinbay, Demet; Tureli, Akif Emre (2007-01-01)
Killer proteins that are produced and secreted into the environment by certain yeast strains are considered as promising antifungal agents. In this study, in vitro activity of Pichia anomala NCYC 434 (K5) killer protein, panomycocin, which is a 49 kDa glycoprotein with an exo-beta-1,3-glucanase activity was tested against 41 isolates of dermatophytes. Minimum inhibitory concentrations (MICs) were determined by a broth microdilution method based on the reference document M38-A of Clinical and Laboratory Stan...
The regulatory effect of CcaR activator on the cephamycin C gene cluster of streptomyces clavuligerus
Kurt, Aslıhan; Özcengiz, Gülay; Department of Biology (2011)
Streptomyces clavuligerus produces industrially important secondary metabolites such as cephamycin C (a β-lactam antibiotic) and clavulanic acid (a potent β-lactamase inhibitor). Cephamycin C is active against penicillin-resistant bacteria due to presence of methoxyl group in C-7 position of cephalosporin nucleus. Clavulanic acid is prescribed in combination with β-lactams for treatment of various bacterial infections. Cephamycin C and clavulanic acid gene clusters form β-lactam supercluster in S. clavulige...
In vitro evaluation of zoledronic acid resistance developed in MCF-7 cells
Kars, Meltem Demirel; Iseri, Ozlem Darcansoy; Ural, Ali Ugur; Gündüz, Ufuk (2007-11-01)
Background: Zoledronic acid is an important osteotropic compound used in combination with anticancer agents to reduce the incidence of hypercalcemia and skeletal morbidity in patients with advanced breast cancer and bone metastases. Ineffectiveness of anticancer drugs during chemotherapy is a frequently observed situation in cancer chemotherapy. The resistance of tumor cells to more than one cytotoxic drugs is defined as multidrug resistance. Drug resistance may be caused by altered gene expression levels a...
In vivo distribution of beta 2 glycoprotein I under various pathophysiologic conditions
Agostinis, Chiara; BİFFİ, Stefania; GARROVO, Chiara; DURİGUTTO, Paolo; LORENZON, Andrea; Bek, Alpan; Bulla, Roberta; Grossi, Claudia; Borghi, Maria O.; Meroni, PierLuigi; Tedesco, Francesco (2011-10-13)
In vitro studies have documented beta 2 glycoprotein I (beta 2GPI) binding to endothelial cells (ECs) and trophoblast using antiphospholipid antibodies. The in vivo binding of beta 2GPI to these cells and the conditions that favor their interaction have not been investigated. We analyzed the in vivo distribution of cyanine 5.5-labeled beta 2GPI in mice and evaluated the effect of pregnancy and circulating antibodies on its tissue localization. The signal was detected in the liver by whole body scan and ex v...
The effect of cysteine-43 mutation on thermostability and kinetic properties of citrate synthase from Thermoplasma acidophilum
Kocabıyık, Semra; Russel, RJM; Danson, MJ; Hough, DW (Elsevier BV, 1996-07-05)
In this study, we have substituted serine-43 by cysteine in the recombinant citrate synthase from a moderately thermophilic Archaeon Thermoplasma acidophilum, for site-specific attachment of labels and have investigated the effects of this mutation on the biochemical properties and thermal stability of the enzyme. Both wild-type and the mutant enzymes were purified to homogenity using affinity chromatography on Matrex Gel Red A. The mutant Thermoplasma citrate synthase is very similar to wild-type citrate s...
Citation Formats
S. Arikan, B. Sancak, and G. Hascelik, “In vitro activity of caspofungin compared to amphotericin B, fluconazole, and itraconazole against Candida strains isolated in a Turkish University Hospital,” MEDICAL MYCOLOGY, pp. 171–178, 2005, Accessed: 00, 2020. [Online]. Available: