ROLE OF CELLS IN FREEZING-INDUCED CELL-FLUID MATRIX INTERACTIONS WITHIN ENGINEERED TISSUES

Role of Cells in Freezing-Induced Cell-Fluid-Matrix Interactions Within Engineered Tissues Seawright, Angela; Özçelikkale, Altuğ; Dutton, Craig; Han, Bumsoo (ASME International, 2013-09-01) During cryopreservation, ice forms in the extracellular space resulting in freezing-induced deformation of the tissue, which can be detrimental to the extracellular matrix (ECM) microstructure. Meanwhile, cells dehydrate through an osmotically driven process as the intracellular water is transported to the extracellular space, increasing the volume of fluid for freezing. Therefore, this study examines the effects of cellular presence on tissue deformation and investigates the significance of intracellular w...
Role of STRO-1 sorting of porcine dental germ stem cells in dental stem cell-mediated bone tissue engineering Pekozer, Gorke Gurel; Ramazanoglu, Mustafa; Schlegel, Karl Andreas; Kök, Fatma Neşe; KÖSE, GAMZE (Informa UK Limited, 2018-01-01) Stem cells of dental origin emerged as a new source for the regeneration of tissues with advantages mainly including non-invasive collection procedures and lack of ethical contraversies with their harvest or use. In this study, porcine TGSCs (pTGSCs) were isolated from mandibular third molar tooth germs of 6-month-old domestic pigs. This is the first study that reports the isolation and characterization of TGSCs from porcine third molars and their differentiation depending on STRO-1 expression. PTGSCs were ...
Role of biochar-based free radicals in immobilization and speciation of metals in the contaminated soil-plant environment Rashid, Muhammad Saqib; Liu, Guijian; Yousaf, Balal; Hamid, Yasir; Rehman, Abdul; Arif, Muhammad; Ahmed, Rafay; Song, Yu; Ashraf, Aniqa (2023-01-01) The structure of biochar produced at various pyrolysis temperatures influences metal geochemical behavior. Here, the impact of wheat straw-derived biochar (300, 500, and 700 degrees C) on the immobilization and trans-formation of metals in the contaminated soil-plant system was assessed. The findings of the sequential extraction revealed that biochar additives had a substantial influence on the speciation of Cr, Ni, Pb, and Zn in the contaminated soil. The lowest F1 (exchangeable and soluble fraction) + F2 ...
Role of intracellular poroelasticity on freezing-induced deformation of cells in engineered tissues Ghosh, Soham; Özçelikkale, Altuğ; Dutton, J. Craig; Han, Bumsoo (The Royal Society, 2016-10-01) Freezing of biomaterials is important in a wide variety of biomedical applications, including cryopreservation and cryosurgeries. For the success of these applications to various biomaterials, biophysical mechanisms, which determine freezing-induced changes in cells and tissues, need to be well understood. Specifically, the significance of the intracellular mechanics during freezing is not well understood. Thus, we hypothesize that cells interact during freezing with the surroundings such as suspension medi...
Role of planktonic bacteria in biodegradation of fish-exuded kairomone in laboratory bioassays of diel vertical migration Beklioglu, M; Gözen, Ayşe Gül; Zorlu, P; Ay-Zog, D (Schweizerbart, 2006-01-01) Daphnia, freshwater crustaceans that graze algae, often rely on predatory chemical cues termed kairomones as signals for predator-avoidance. Using laboratory bioassays, we studied how planktonic bacteria may modify kairomone activity. We measured the amplitude of diel vertical migration (DVM) of Daphnia pulex DE GEER among treatments with different amounts of bacteria. We used temperature incubation to increase bacterial densities and filtration to reduce abundance. Daphnids exposed to fish cue (F) and filt...

Citation Formats

A. Seawright, A. Özçelikkale, J. C. Dutton, and B. Han, “ROLE OF CELLS IN FREEZING-INDUCED CELL-FLUID MATRIX INTERACTIONS WITHIN ENGINEERED TISSUES,” 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/69400.