Cephamycin C overproduction upon releasing relaxing precursor flux by genetic engineering and medium formulation.

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2008-08-05

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Özcengiz, Gülay

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https://hdl.handle.net/11511/72498

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Cephamycin C production by Streptomyces clavuligerus mutants impaired in regulation of aspartokinase Zeyniyev, Araz; Özcengiz, Gülay; Department of Biotechnology (2006) Aspartokinase is the first enzyme of the aspartate family amino acids biosynthetic pathway. Cephamycin C is a β-lactam antibiotic produced as a secondary metabolite via the enzymatic reactions in the lysine branch of this pathway in Streptomyces clavuligerus. The aspartokinase activity of S. clavuligerus is under concerted feedback inhibition by two of the end product amino acids, lysine plus threonine. It is also known that carbon flow through the lysine branch of the aspartate pathway is rate limiting ste...
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Celecoxib-loaded liposomes: effect of cholesterol on encapsulation and in vitro release characteristics DENİZ, Asli; Sade, Asli; Severcan, FERİDE; Keskin, Dilek; Tezcaner, Ayşen; Banerjee, Sreeparna (2010-10-01) CLX (celecoxib) is a highly hydrophobic non-steroidal anti-inflammatory drug with high plasma protein binding. We describe here the encapsulation of CLX in MLVs (multilamellar vesicles) composed of DSPC (1,2-distearoyl-sn-glycero-3-phosphocholine) and variable amounts of cholesterol. The effects of cholesterol content on liposome size, percentage drug loading and in vitro drug release profiles were investigated. Differential scanning calorimetry and FTIR (Fourier-transform infrared) spectroscopy were used t...
Celecoxib reduces fluidity and decreases metastatic potential of colon cancer cell lines irrespective of COX-2 expression Sade, Asli; Tuncay, Seda; Cimen, Ismail; Severcan, Feride; Banerjee, Sreeparna (Portland Press Ltd., 2012-02-01) CLX (celecoxib), a selective COX-2 (cyclo-oxygenase-2) inhibitor, has numerous pleiotropic effects on the body that may be independent of its COX-2 inhibitory activity. The cancer chemopreventive ability of CLX, particularly in CRC (colorectal cancer), has been shown in epidemiological studies. Here we have, for the first time, examined the biophysical effects of CLX on the cellular membranes of COX-2 expressing (HT29) and COX-2 non-expressing (SW620) cell lines using ATR-FTIR (attenuated total reflectance ...
Celecoxib Nanoformulations with Enhanced Solubility, Dissolution Rate, and Oral Bioavailability: Experimental Approaches over In Vitro/In Vivo Evaluation Arslan, Aslıhan; Yet, Barbaros; NEMUTLU, EMİRHAN; AKDAĞ ÇAYLI, YAĞMUR; EROĞLU, HAKAN; ÖNER, LEVENT (2023-02-01) Celecoxib (CXB) is a Biopharmaceutical Classification System (BCS) Class II molecule with high permeability that is practically insoluble in water. Because of the poor water solubility, there is a wide range of absorption and limited bioavailability following oral administration. These unfavorable properties can be improved using dry co-milling technology, which is an industrial applicable technology. The purpose of this study was to develop and optimize CXB nanoformulations prepared by dry co-milling techn...

Citation Formats

G. Özcengiz, “Cephamycin C overproduction upon releasing relaxing precursor flux by genetic engineering and medium formulation.,” 2008, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/72498.