Simultaneous Determination of Immunosuppressive Drugs from Whole Blood by CoatedBlade Spray Ionization-Mass Spectrometry

2017-03-05
Tascon, Marcos
Boyacı, Ezel
Gómez-Ríos, German A
Pawliszyn, Janusz
Poole, Justen J
Reyes-Garcés, Nathaly
Coated Blade Spray (CBS) ionization was conceived as a fast and simple sampling/sample-preparation and ionization method for qualitative/quantitative mass spectrometry (MS) analysis of drugs of abuse, pharmaceuticals, and other small molecules from urine, blood, and other biofluids. This study describes the use of the CBS for the quantitation of immunosuppressive drugs from 100 µL of blood samples, focusing specifically on the simultaneous quantitation of everolimus, tacrolimus, sirolimus, and cyclosporine-A. These drugs are characterized for having a narrow therapeutic range (e.g. 2-15 ng mL-1, 5-15 ng mL-1, 6-8 ng mL-1, and 100-350 ng mL-1 for tacrolimus (TAC), sirolimus (SIR), everolimus (EVR) and cyclosporine A (CycA), respectively); therefore, continuous monitoring to avoid either subtherapeutic or toxic effect is always required. Our results showed rewarding limits of quantitation (1 ng mL-1 for EVR/SIR/TAC, and 10 ng mL-1 for CycA) as well as good accuracy (• 90 %) and linearity (R2>0.99) over the range evaluated for all the compounds (1-50 ng/mL for EVR/SIR/TAC, and 10-500 ng mL-1 for CycA). The precision of the method was below 15% in all the cases and the bias for three different validation points ranged from 2% to 10%. No matrix effects were observed when evaluating this methodology at different hematocrit levels. By using a high-throughput autosampler, a total analysis time of less than 3 minutes can be attained per sample.
Citation Formats
M. Tascon, E. Boyacı, G. A. Gómez-Ríos, J. Pawliszyn, J. J. Poole, and N. Reyes-Garcés, “Simultaneous Determination of Immunosuppressive Drugs from Whole Blood by CoatedBlade Spray Ionization-Mass Spectrometry,” presented at the Pittcon 2017, (5-09 Mart 2017), Şikago, Amerika Birleşik Devletleri, 2017, Accessed: 00, 2021. [Online]. Available: https://pittcon.org/2017/Pittcon-2017-Technical-Program.pdf.