Cloning, characterization and heterologous expression of mesodiaminopimelic acid decarboxylase (lysA) gene from Streptomyces clavuligerus

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2006-07-04

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Özcengiz, Gülay

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https://hdl.handle.net/11511/75575

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Cloning, characterization and heterologous expression of the aspartokinase and aspartate semialdehyde dehydrogenase genes from the cephamycin C producer streptomyces clavuligerus Tunca, Sedef; Özcengiz, Gülay; Piret, Jacqueline; Department of Biotechnology (2002) The carbon flow through the lysine branch of aspartate pathway is a rate limiting step in the formation of cephamycin C, a broad spectrum P-lactam antibiotic produced by Streptomyces clavuligerus. In this study, the ask m(aspartokinase) and asd (aspartate semialdehyde dehydrogenase) genes which encode the enzymes catalyzing the first two steps of aspartate pathway were cloned and characterized as the first time from S. clavuligerus NRRL 3585. This was accomplished by constructing four mini-libraries of S. c...
Cloning and characterization of Streptomyces clavuligerus meso-diaminopimelate decarboxylase (lysA) gene Yağcıoğlu, Çiğdem; Özcengiz, Gülay; Department of Biology (2004) In Streptomyces clavuligerus, the route to the biosynthesis of a-aminoadipic acid (a-AAA) represents an important primary metabolic pathway providing carbon flux to the synthetases of antibiotic formation. This carbon flow comes through the lysine-specific branch of the aspartate pathway and is rate limiting in the formation of cephamycin C, a second generation cephalosporin produced by this organism. In this study, the lysA gene which encodes for an important key enzyme of aspartate pathway; meso-diaminopi...
Cloning, characterization and heterologous expression of the aspartokinase and aspartate semialdehyde dehydrogenase genes of cephamycin C-producer Streptomyces clavuligerus Tunca, S; Yilmaz, EI; Piret, J; Liras, P; Özcengiz, Gülay (Elsevier BV, 2004-09-01) Carbon flow through the lysine branch of the aspartate biosynthetic pathway is a rate-limiting step in the formation of cephamycin C, a broad spectrum P-lactam antibiotic produced by Streptomyces clavuligerus. In this study, genes which encode the enzymes catalyzing the first two steps of the aspartate pathway, ask (aspartokinase) and asd (aspartate semialdehyde dehydrogenase), in S. clavuligerus NRRL 3585 were cloned and sequenced. Nucleotide sequencing and codon preference analysis revealed three complete...
Cloning and characterization of trehalose-6-phosphate synthase gene from rhizopus oryzae Özer Uyar, Gülsüm Ebru; Hamamcı, Haluk; Department of Biotechnology (2009) In many organisms, trehalose protects against several environmental stresses, such as heat, desiccation and salt, probably by stabilizing protein structures and lipid membranes. Trehalose-6-phosphate synthase 1 (TPS1) is a subunit of trehalose synthase complex in fungi; it plays a key role in the biosynthesis of trehalose. In this study, a TPS1 gene fragment in R. oryzae was cloned successfully by PCR with primers designed according to eight hypothetical proteins found from BLAST search which was performed ...
Cloning, sequencing, and characterization of CYP1A1 cDNA from leaping mullet (Liza saliens) liver and implications for the potential functions of its conserved amino acids Sen, A; Hu, CH; Urbach, E; Wang-Buhler, JL; Yang, YH; Arinc, E; Buhler, DR (Wiley, 2001-01-01) A 2,037 bp CYP1A1 cDNA (GenBank AF072899) was cloned through screening of a lambda ZipLox cDNA library constructed from the liver of a leaping mullet (Liza saliens) fish captured from Izmir Bay on the Aegean coast of Turkey using rainbow trout CYP1A1 cDNA as a probe. This clone has a 130 bp 5'-flanking region, a 1,563 bp open reading frame (ORF) encoding a 521-amino acid protein (58,972 Da), and a 344 bp 3'-untranslated region without a poly (A) tail. Alignment of the deduced amino acids of CYP1A1 cDNAs sho...

Citation Formats

G. Özcengiz, “Cloning, characterization and heterologous expression of mesodiaminopimelic acid decarboxylase (lysA) gene from Streptomyces clavuligerus,” 2006, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/75575.