Integration of a regulatory gene into the chromosome of an industrial Streptomyces clavuligerus strain for enhanced clavulanic acid biosynthesis

2009-09-27

Suggestions

Integration of clavaminate synthase 2 gene into the chromosome of an industrial strain of Streptomyces Clavuligerus for enhanced clavulanic acid production
Vanlı, Güliz; Özcengiz, Gülay; Özkan, Melek; Department of Biotechnology (2010)
Streptomyces clavuligerus is a gram-positive, filamentous bacterium which has a great ability to produce secondary metabolites including isopenicillin N, cephamycin C and a beta-lactamase inhibitor clavulanic acid. Clavulanic acid (CA) which is a bicyclic beta-lactam, inhibits most of class A beta-lactamases by binding irreversibly to the serine hydroxyl group at the active center of beta-lactamases and resulting in the stable acyl-enzyme complexes. Clavaminate synthase (CAS) is one of the best characterize...
Integration of a Glutamate Sensitive Genetically Encoded Sensor Protein into Photocrosslinkable Hydrogel Optrodes
Kahyaoğlu, Leyla Nesrin (2016-01-01)
Immobilization into 3D matrices stabilizes proteins in comparison to flat planar surfaces and facilitates the study of the biomolecular interactions as well as integration into optrodes for cell physiology. Photocrosslinkable hydrogels have received significant attention in recent years as they provide not only a highly hydrophilic 3D environment to promote protein stabilization and its interactions with analyte molecules, but enable optically addressable patterning for spatial control of protein localizati...
Integration of a Genetically Encoded Calcium Molecular Sensor into Photopolymerizable Hydrogels for Micro-Optrode-Based Sensing
Kahyaoğlu, Leyla Nesrin; Park, Joon Hyeong; Rickus, Jenna L. (American Chemical Society (ACS), 2017-09-20)
Genetically encoded molecular-protein sensors (GEMS) are engineered to sense and quantify a wide range of biological substances and events in cells, in vitro and even in vivo with high spatial and temporal resolution. Here, we aim to stably incorporate these proteins into a photopatternable matrix, while preserving their functionality, to extend the application of these proteins as spatially addressable optical biosensors. For this reason, we examined the fabrication of 3D hydrogel microtips doped with a ge...
Transfer of stripe rust resistance gene Yr26 to Turkish wheats using microsatellite markers
Yildirim, A; Karadag, Y; Sakin, MA; Gokmen, S; Kandemir, N; Akkaya, Mahinur; Yildirim, F (2004-01-01)
Stripe rust (yellow rust) is one of the most devastating diseases of wheat worldwide. The most effective control method of stripe rust is the use of resistance genes. A very effective stripe rust resistance gene, Yr26, originating from Triticum turgidum L., was transferred into two common wheat (Triticum aestivum L.) cultivars of Turkey through backcrossing using closely linked microsatellite markers. A 6VS/6AL translocation line (92R149) was used as the source of Yr26 because this gene was originally thoug...
Transformation of nicotiana tabacum plants with na+/h+ antiporter (atnhx1) gene isolated from arabidopsis thaliana for evaluation of salt tolerance
Aysin, Ferhunde; Erson Bensan, Ayşe Elif; Department of Biology (2007)
Large, membrane-bound vacuoles of plant cells are suitable organelles for the compartmentation of ions. These vacuoles contain Na+/H+ antiporters for movement of Na+ within the organelle in exchange for H+. They provide an efficient mechanism to prevent the occurance of detrimental outcomes of Na+ accumulation in the cytosol. Identification of AtNHX1 gene that confers resistance to salinity by expressing a Na+/H+ antiport pump facilitates the understanding of the salt stress tolerance mechanisms of plants. ...
Citation Formats
G. Özcengiz, “Integration of a regulatory gene into the chromosome of an industrial Streptomyces clavuligerus strain for enhanced clavulanic acid biosynthesis,” 2009, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/76762.