Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Early Stage Detection of Tobacco Mosaic Virus in Tomato Seeds by NMR Relaxometry
Date
2015-08-12
Author
Unal, Kubra
Alpas, Hami
Cevik, Bayram
Aktas, Hakan
Oztop, Mecit
Metadata
Show full item record
Item Usage Stats
36
views
0
downloads
Cite This
Because of rapidly increasing population and limited agricultural areas, it becomes compulsory to fight against viral contamination that disrupts effective agricultural production. In this study, use of NMR Relaxometry is investigated to see the effect of salt diffusion and to detect seeds, which had been infected by virus. Tobacco mosaic virus (TMV) infected tomato is chosen since tomato has a high commercial value in sector. T2 relaxation times were measured by using low resolution NMR Relaxometry. Tomato seeds were diluted with distilled water and with 10%, 20%, 30% NaCI solutions and measurements were done by 15min intervals. T2 values of diluted virus free seeds has dropped to 278 ms from 372 ms after 1 day waiting and has dropped to 189 ms after 2 days. In order to distinguish signals coming from different compartments of seed, relaxation spectra were obtained. At seeds diluted with distilled water, it was observed that T2 of peaks have become closer to each other after 1 day due to diffusion of water into cells and becoming relative water by time. In infected seeds, merging of peaks and decrease in T2 were observed after 1 day. 3 peaks were observed at relaxation spectra after 2 day. Presence of viruses in seeds causes changes in cell structure which can directly be observed by relaxation spectra. Seeds diluted with NaCI solution revealed higher T2 values because of lysis of cell wall and diffusion of more water into cell. These results have proved that NMR Relaxometry can accurately be used in differentiation of infected seeds.
URI
https://hdl.handle.net/11511/77463
Collections
Department of Food Engineering, Conference / Seminar
Suggestions
OpenMETU
Core
Development of salt resistant transgenic plants by using TaNHX1 and TaSTR genes
Kavas, Musa; Yücel, Ayşe Meral; Öktem, Hüseyin Avni; Department of Biology (2011)
Soil salinity negatively affects agricultural production in Turkey by decreasing the yield and quality. Direct introduction of stress related genes by genetic engineering is one of the most rapid approaches to develop stress tolerant crops. In this study, TaNHX1 gene was isolated from bread wheat and three different local wheat cultivars were transformed with overexpression vectors containing TaNHX1 gene by using Agrobacterium-mediated and particle bombardment gene transfer techniques. Immature embryo and i...
The Evaporative Stress Index as an indicator of agricultural drought in Brazil: An assessment based on crop yield impacts
Anderson, Martha C.; Zolin, Cornelio A.; Sentelhas, Paulo C.; Hain, Christopher R.; Semmens, Kathryn; Yılmaz, Mustafa Tuğrul; Gao, Feng; Otkin, Jason A.; Tetrault, Robert (2016-03-01)
To effectively meet growing food demands, the global agronomic community will require a better understanding of factors that are currently limiting crop yields and where production can be viably expanded with minimal environmental consequences. Remote sensing can inform these analyses, providing valuable spatiotemporal information about yield-limiting moisture conditions and crop response under current climate conditions. In this paper we study correlations for the period 2003-2013 between yield estimates f...
Microarray based expression profiling of barley under boron stress and cloning of 3H boron tolerance gene
Öz, Mehmet Tufan; Eyidoğan, Füsun; Öktem, Hüseyin Avni; Department of Biotechnology (2012)
Both deficiency and toxicity of the essential micronutrient boron (B) lead to reduced crop yield in agriculture. However, our understanding of the molecular responses of plants under B stresses to tackle the yield loss is limited. Therefore, in the present study, transcriptional alterations in sensitive and tolerant barley cultivars under B deficiency and toxicity were investigated in order to reveal the molecular responses. Transcriptomes were monitored at seedling stage by global expression profiling usin...
Identification and cloning of genes induced and / or repressed upon treatments of wheat plants (avocet s) with BTH, BABA and Trichoderma Harzianum Raifi KRL-AG2
Al-Asbahi, Adnan; Akkaya, Mahinur S.; Department of Biotechnology (2006)
One of the major problems concerning the production of food crops is the controlling of plant diseases to maintain the high quality and yield. Wheat diseases are caused by parasitic bacteria, fungi and viruses that are a major hazard in wheat production. Therefore, understanding of any resistance mechanism is prerequisite for the successful utilization of wheat crop species in modern agriculture. The phenomenon of induced resistance by fungi, bacteria, microbial elicitors and chemicals has been investigated...
Early yield estimation by photosynthetic pigment abundances using landsat 8 image series
Özcan, Ayşenur; Süzen, Mehmet Lütfi; Leloğlu, Uğur Murat; Department of Geodetic and Geographical Information Technologies (2020-10-13)
Timely estimation of crop yields is critical for monitoring global food production by international organizations as well as governments, farmers and the private sector dealing with storage, import and export of crops and associated products. Satellite remote sensing has the capability to provide near real-time information on a global scale. Combining satellite data and soft computing techniques to predict crop yields is a very effective strategy for continually forecasting crop yields. This thesis pr...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
K. Unal, H. Alpas, B. Cevik, H. Aktas, and M. Oztop, “Early Stage Detection of Tobacco Mosaic Virus in Tomato Seeds by NMR Relaxometry,” 2015, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/77463.