Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Transcriptional analysis of the effect of ccaR regulatory gene on cephamycin C biosynthesis by Streptomyces clavuligerus
Date
2009-09-27
Author
Özcengiz, Gülay
Metadata
Show full item record
Item Usage Stats
59
views
0
downloads
Cite This
URI
https://hdl.handle.net/11511/80559
Collections
Unverified, Conference / Seminar
Suggestions
OpenMETU
Core
Transcriptional analysis of hydrogenase genes in rhodobacter sphaeroides O.U.001
Doğrusöz, Nihal; Gündüz, Ufuk; Department of Biology (2004)
In photosynthetic non-sulphur bacteria, hydrogen production is catalyzed by nitrogenases and hydrogenases. Hydrogenases are metalloenzymes that are basically classified into: the Fe hydrogenases, the Ni-Fe hydrogenases and metal-free hydrogenases. Two distinct Ni-Fe hydrogenases are described as uptake hydrogenases and bidirectional hydrogenases. The uptake hydrogenases are membrane bound dimeric enzymes consisting of small (hupS) and large (hupL) subunits, and are involved in uptake and the recycling of hy...
Transcriptional engineering of the glyceraldehyde-3-phosphate dehydrogenase promoter for improved heterologous protein production in Pichia pastoris
Ata, Ozge; Prielhofer, Roland; Gasser, Brigitte; Mattanovich, Diethard; Çalık, Pınar (2017-10-01)
The constitutive glyceraldehyde-3-phosphate dehydrogenase promoter (P-GAP), which is one of the benchmark promoters of Pichia pastoris, was analyzed in terms of putative transcription factor binding sites. We constructed a synthetic library with distinct regulatory properties through deletion and duplication of these putative transcription factor binding sites and selected transcription factor (TF) genes were overexpressed or deleted to understand their roles on heterologous protein production. Using enhanc...
Transcriptional Profiling of Hydrogen Production Metabolism of Rhodobacter capsulatus under Temperature Stress by Microarray Analysis
Gurgan, Muazzez; Erkal, Nilufer Afsar; Ozgur, Ebru; Gündüz, Ufuk; Eroglu, Inci; Yücel, Ayşe Meral (2015-06-01)
Biohydrogen is a clean and renewable form of hydrogen, which can be produced by photosynthetic bacteria in outdoor large-scale photobioreactors using sunlight. In this study, the transcriptional response of Rhodobacter capsulatus to cold (4 degrees C) and heat (42 degrees C) stress was studied using microarrays. Bacteria were grown in 30/2 acetate/glutamate medium at 30 degrees C for 48 h under continuous illumination. Then, cold and heat stresses were applied for two and six hours. Growth and hydrogen prod...
Transcriptional engineering of GAP promoter for improved recombinant protein production by Pichia pastoris
Ata, Özge; Çalık, Pınar; Mattanovich, Diethard; Department of Biotechnology (2017)
The objective of this PhD thesis is to enhance the expression strength of glyceraldehyde-3-phosphate dehydrogenase promoter (PGAP) for improved recombinant protein (r-protein) production through modifying the transcription factors (TF) that regulate the functioning of PGAP by transcriptional engineering. PGAP was analyzed in terms of putative TF binding sites. A synthetic library was constructed with distinct regulatory properties through deletion and duplication of these putative transcription factor bindi...
Transcriptional engineering of pichia pastoris alcohol dehydrogenase 2 and alcohol oxidase 1 promoters for recombinant protein production
Gündüz Ergün, Burcu; Çalık, Pınar; Mattanovich, Diethard; Department of Biotechnology (2018)
The aim of the Ph.D. thesis was designing novel engineered promoter variants (NEPVs) to obtain strong ethanol regulated promoters for improved recombinant protein (r-protein) production, and to clarify the role of certain transcription factors (TFs) on the regulation of P. pastoris PADH2 and PAOX1. Promoter architecture of PADH2 was redesigned by nucleosome optimization and modification of transcription factor binding sites and, the induction mechanism of PAOX1 was changed to ethanol as sole carbon and ener...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
G. Özcengiz, “Transcriptional analysis of the effect of ccaR regulatory gene on cephamycin C biosynthesis by Streptomyces clavuligerus,” 2009, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/80559.