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Effects of Metals on Elastase from Pseudomonas aeruginosa SES-938-1
Date
1995-10-01
Author
Kocabıyık, Semra
Ergin, Ezgi
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Among the numerous virulance factors produced byPseudomonas aeruginosa, elastase is the one most often associated with pathogenesis. In this study, effects of various metal ions on elastase from a new isolate ofP. aeruginosa (Strain SES-938-1) was investigated. Crude elastase was prepared from culture supernatant via salting out by ammonium sulfate, and then desalting and concentrating the sample using a centricon microconcentrator. Activities were measured at 450 nm usingN-succinyl-L-(ala)3-p-nitroanilide as the substrate. The metal chelating agents EDTA and EGTA inhibited thePseudomonas elastase, which shows that the enzyme is a typical metalloproteinase. At a 10-mM concentration, Mn2+, Ni2+, and Zn2+ strongly inhibited the elastase, whereas Mg2+ effect was negligable. There was a gradual decrease in the enzyme activity in accordance with an increase in the concentration of metal ions.
URI
https://hdl.handle.net/11511/81779
https://link.springer.com/article/10.1007%2FBF02789146
Journal
Biological Trace Element Research
Collections
Department of Biology, Article
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S. Kocabıyık and E. Ergin, “Effects of Metals on Elastase from Pseudomonas aeruginosa SES-938-1,”
Biological Trace Element Research
, pp. 25–31, 1995, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/81779.