Effects of Metals on Elastase from Pseudomonas aeruginosa SES-938-1

1995-10-01
Kocabıyık, Semra
Türkoğlu, Suna
Ergin, Ezgi
Among the numerous virulance factors produced byPseudomonas aeruginosa, elastase is the one most often associated with pathogenesis. In this study, effects of various metal ions on elastase from a new isolate ofP. aeruginosa (Strain SES-938-1) was investigated. Crude elastase was prepared from culture supernatant via salting out by ammonium sulfate, and then desalting and concentrating the sample using a centricon microconcentrator. Activities were measured at 450 nm usingN-succinyl-L-(ala)3-p-nitroanilide as the substrate. The metal chelating agents EDTA and EGTA inhibited thePseudomonas elastase, which shows that the enzyme is a typical metalloproteinase. At a 10-mM concentration, Mn2+, Ni2+, and Zn2+ strongly inhibited the elastase, whereas Mg2+ effect was negligable. There was a gradual decrease in the enzyme activity in accordance with an increase in the concentration of metal ions.
Citation Formats
S. Kocabıyık, S. Türkoğlu, and E. Ergin, “Effects of Metals on Elastase from Pseudomonas aeruginosa SES-938-1,” pp. 25–31, 1995, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/81779.