Phaseguide assisted liquid lamination for magnetic bead-based assays

Phurimsak, Chayakom
Yıldırım, Ender
Trietsch, Sebastiaan J
Hankemeier, Thomas
Tarn, Mark D
Pamme, Nicole
Vulto, Paul
We demonstrate a simple, pump-free platform for performing rapid magnetic bead-based processes via their transfer through sequentially laminated liquid streams, made possible by the use of phaseguide technology. We have applied this strategy to two on-chip assays: (i) a streptavidin-biotin binding assay, and (ii) a sandwich immunoassay for the detection of C-reactive protein (CRP). Here, functionalized magnetic beads were pulled through alternating lanes of reagents and buffer solution, allowing multiple binding and washing processes to be reduced into a single step, significantly shortening procedural times compared to conventional multi-step bead-based assays.
7th International Conference on MiniaturizedSystems for Chemistry and Life Sciences27-31 October 2013


Phaseguide assisted liquid lamination for magnetic particle-based assays
Phurimsak, Chayakom; Yıldırım, Ender; Tarn, Mark D.; Trietsch, Sebaastian J; Hankemeier, Thomas; Pamme, Nicole; Vulto, Paul (Royal Society of Chemistry (RSC), 2014-01-01)
We have developed a magnetic particle-based assay platform in which functionalised magnetic particles are transferred sequentially through laminated volumes of reagents and washing buffers. Lamination of aqueous liquids is achieved via the use of phaseguide technology; microstructures that control the advancing air-liquid interface of solutions as they enter a microfluidic chamber. This allows manual filling of the device, eliminating the need for external pumping systems, and preparation of the system requ...
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Citation Formats
C. Phurimsak et al., “Phaseguide assisted liquid lamination for magnetic bead-based assays,” presented at the 7th International Conference on MiniaturizedSystems for Chemistry and Life Sciences27-31 October 2013, Freiburg, Germany, 2013, Accessed: 00, 2021. [Online]. Available: