Phaseguide assisted liquid lamination for magnetic bead-based assays

Date

2013-10-27

Author

Phurimsak, Chayakom
Yıldırım, Ender
Trietsch, Sebastiaan J
Hankemeier, Thomas
Tarn, Mark D
Pamme, Nicole
Vulto, Paul

Metadata

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We demonstrate a simple, pump-free platform for performing rapid magnetic bead-based processes via their transfer through sequentially laminated liquid streams, made possible by the use of phaseguide technology. We have applied this strategy to two on-chip assays: (i) a streptavidin-biotin binding assay, and (ii) a sandwich immunoassay for the detection of C-reactive protein (CRP). Here, functionalized magnetic beads were pulled through alternating lanes of reagents and buffer solution, allowing multiple binding and washing processes to be reduced into a single step, significantly shortening procedural times compared to conventional multi-step bead-based assays.

Subject Keywords

Magnetic beads, C-reactive protein , Immunoassays, Liquid lamination, Phaseguides

URI

https://hdl.handle.net/11511/82769
https://www.rsc.org/images/loc/2013/PDFs/Papers/386_0905.pdf

DOI

https://doi.org/https://doi.org/10.1039/C4LC00139G

Conference Name

7th International Conference on MiniaturizedSystems for Chemistry and Life Sciences27-31 October 2013

Collections

Department of Mechanical Engineering, Conference / Seminar

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Citation Formats

C. Phurimsak et al., “Phaseguide assisted liquid lamination for magnetic bead-based assays,” presented at the 7th International Conference on MiniaturizedSystems for Chemistry and Life Sciences27-31 October 2013, Freiburg, Germany, 2013, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/82769.