Bacilysin is a small, fine-tuning effector of mother cell compartment-specific σE and σK regulons in Bacillus subtilis

2017-06-11
Özcengiz, Gülay
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https://hdl.handle.net/11511/84623
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Biosynthesis of dipeptide antibiotic bacilysin by a partially purified enzyme prepared from Bacillus subtilis PY79 was studied. Cell material was desintegrated by treatment with lysozyme and sonication and the extract was subjected to ammonium sulfate fractionation. Bacilysin-synthesizing enzyme activity was precipitated between 40% to 70% ammonium sulfate saturation. In vitro enzymatical synthesis of bacilysin was confirmed by performing thin layer chromatographic comparison of the antibiotic formed with t...
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Bacilysin-negative(bac-) strain NG79 was found to be oligosporogenous. When compared with the parental strain, it was 200-300 times less resistant to heat, chloroform, and lysozyme treatments, and the spores contained considerably less dipicolinate. When NG79 was transduced, the oligosporogenous phenotype was found to be cured in all the transductants tested. External addition of bacilysin to the cultures of this strain markedly improved each measure of spore quality. The time of its addition determined...
Bacillus subtilis overproduces industrially important extracellular enzymes upon the targeted deletion of bacilysin biosynthetic operon
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Bacilysin being produced by Bacillus subtilis is the smallest peptide antibiotic ever known. It is composed of an N-terminal l-alanine and a modified amino acid at its C-terminal, namely anticapsin. bacABCDEF operon and a monocistronic gene bacG are functional for bacilysin production in the organism, bacABCDFG being needed for the flux from prephenate to anticapsin and then to mature bacilysin while bacE gene within the operon is involved in resistance of the producer by pumping bacilysin out of the cell. ...
BACILYSIN PRODUCTION BY BACILLUS-SUBTILIS - EFFECTS OF BACILYSIN, PH AND TEMPERATURE
Özcengiz, Gülay (Springer Science and Business Media LLC, 1991-01-01)
The dipeptide antibiotic bacilysin, when added externally to the early exponential-phase cultures, markedly limited its own synthesis. It was shown in cell-free extracts that the feedback effect does not involve the inhibition of bacilysin synthetase, the enzyme catalyzing bacilysin formation. We also studied pH and temperature dependence of bacilysin production. Production was highest at about pH 6.8 and at 25-degrees-C.
Bactericidal and in vitro osteogenic activity of nano sized cobalt-doped silicate hydroxyapatite
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Citation Formats
G. Özcengiz, “Bacilysin is a small, fine-tuning effector of mother cell compartment-specific σE and σK regulons in Bacillus subtilis,” 2017, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/84623.
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