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Metabolic engineering of aspartate pathway for increased production of cephamycin C in Streptomyces clavuligerus
Date
2009-09-27
Author
Özcengiz, Gülay
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https://hdl.handle.net/11511/86041
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Metabolic control sites in the aromatic group amino acid pathway (AAAP) of Bacillus subtilis for L-Phenylalanine (Phe) overproduction were determined; and aiming pathway flux amplification, by cloning the flux controlling gene aroH to a multi-copy plasmid, the impact of single gene cloning on pathway flux distributions were investigated. The branch-point metabolites E4P and PEP+E4P supplied in vitro, enhanced Phe production and well defined perturbations were achieved on the AAAP reactions. The intracellula...
Metabolic engineering of L-phenylalanine pathway in Bacillus subtilis
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The objectives of this thesis are investigation of, i) the effects of oxygen transfer conditions on recombinant protein production in Pichia pastoris strains designed with novel naturally occuring pyruvate kinase (PYK) promoter (PPYK) which is a potential promoter for recombinant protein production under low to moderate oxygen transfer conditions, and ii) influences of engineering with single- and multi- copy genes, in fed-batch fermentation processes. Production of recombinant human growth hormone (rhGH) b...
Metabolic flux analyses for serine alkaline protease production
Çalık, Pınar; Ozdamar, TH (2000-12-01)
The intracellular metabolic fluxes through the central carbon pathways in Bacillus licheniformis in serine alkaline protease (SAP) production were calculated to predict the potential strategies for increasing the performance of the bacilli, by using two optimization approaches, i.e. the theoretical data-based (TDA) and the theoretical data-based capacity (TDC) analyses based on respectively minimum in-vivo SAP accumulation rate and maximum SAP synthesis rate assumptions, at low-, medium, and high-oxygen tra...
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Senturk, B; Çalık, Pınar; Calik, G; Ozdamar, TH (2005-08-01)
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G. Özcengiz, “Metabolic engineering of aspartate pathway for increased production of cephamycin C in Streptomyces clavuligerus,” 2009, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/86041.