Chip-based immunomagnetic separation of human chorionic gonadotropin

Date

2016-09-29

Author

Ahi, Elçin Ezgi
Gümüştaş, Aysen
Çiftçi, Hakan
Çağlayan, M Gökhan
Selbes, Yeşim Somay
Çoğun, Ferah
Yıldırım, Ender
Tamer, Uğur

Metadata

Show full item record

Item Usage Stats

84
views

0
downloads

Human chorionic gonadotropin (hCG) is a glycoprotein, and it can be misused by male athletes. This glycoprotein affects testosterone production and release in the human body. Therefore, the use of this hormone as a performance enhancing drug has been prohibited by the World Anti-Doping Agency [1]. In this study, a microchip, which had developed by our group, was used for the detection of hCG using both colorimetric and spectrofluorometric measurements. For this purpose, gold coated magnetic nanoparticles (MNPs) and gold nanorods (AuNRs) were synthesized and modified using different reagents and specific antibody, respectively [2]. Then they were used to form in-chip sandwich immunoassay with the hCG for colorimetric measurement. The developed microchips consist of four micro-chambers connected to each other by microchannels. The first chamber was filled with modified MNPs, the second and third chambers were filled with modified AuNRs and washing buffer, respectively. The last chamber was filled with 3, 3´, 5, 5´-tetramethylbenzidine (TMB) and H2O2, then used for monitoring the color change. The chip process was performed by moving the MNPs from the first chamber through the microchannels using a magnet. The first step of the process is forming sandwich structure between the MNPs and the horse radish peroxidase (HRP) -labelled AuNRs. The second step includes washing the sandwich structure with the washing buffer. Finally, the enzymatic reaction between the TMB and H2O2 occurs in the presence of HRP in the last chamber, then the color change is observed. To get fluorescence signal, this time the second chamber of the microchip was filled with the europium (Eu) labelled-anti hCG, the third and last chamber was filled with the washing buffer. Finally, measurement was performed in the last chamber following same procedure used in colorimetric detection by a spectrofluorometer equipped with a fiber optic prob.

Subject Keywords

AuNRs, Colorimetric detection, hCG, MNPs, Microchip, Spectrofluorometric measurement

URI

http://aacd2016.comu.edu.tr/announcements/abstract_book_of_aac
https://hdl.handle.net/11511/88196

Conference Name

10th Aegean Analytical Chemistry Days - AACD 2016, 29 September- 2 October 2016

Collections

Department of Mechanical Engineering, Conference / Seminar

Suggestions

OpenMETU
Core

A capillary driven microfluidic chip for SERS based hCG detection Ahi, Elcin Ezgi; TORUL, HİLAL; Zengin, Adem; Sucularli, Ferah; Yıldırım, Ender; Selbes, Yesim; SULUDERE, ZEKİYE; TAMER, UĞUR (2022-01-01) In this study, a capillary driven microfluidic chip-based immunoassay was developed for the determination of Human Chorionic Gonadotropin (hCG) protein, which is prohibited by the World Anti-Doping Agency (WADA). Here, we used antibody modified magnetic metal organic framework nanoparticles (MMOFs) as a capture prob in urine sample. MMOF captured hCG was transferred in a capillary driven microfluidic chip consisting of four chambers, and the interaction of MMOF with gold nanorods labelled with 5,5'-Dithiobi...
Protein characterization of human YPEL2 and YPEL homolog yeast MOH1 Olgun, Çağla Ece; Muyan, Mesut; Department of Molecular Biology and Genetics (2018) 17ß-estradiol (E2), the main circulating estrogen hormone, has an important role in the physiological and pathophysiological regulation of many tissues and organs including breast tissue. Regulation of cell proliferation, differentiation and death in target tissues is mediated by E2. The estrogen receptor (ER), a transcription factor, provides the lasting effect of E2 on cells via regulation of targeting gene expression. Previous microarray and gene expression studies in our laboratory reveal that YPEL2, wh...
Growth model for abdominal aortic aneurysms using longitudinal CT images Akkoyun, Emrah; Acar, Aybar Can; Department of Health Informatics (2020) An Abdominal Aortic Aneurysm (AAA) is diagnosed by an enlargement of the abdominal aorta. The rupture of an AAA, associated with high mortality, is eventually observed if no surgical intervention is performed. Aneurysm repair prior to rupture is thus vital. The decision to intervene is made primarily based on the AAA size measured by a maximum diameter or its growth rate. However, 10 – 24% of aneurysms below the intervention threshold experience rupture in some series. There are many complex interactions in...
Metabolic syndrome and its components among women with polycystic ovary syndrome: a systematic review and meta-analysis Hallajzadeh, Jamal; Khoramdad, Maliheh; Karamzad, Nahid; Almasi-Hashiani, Amir; Janati, Ali; Ayubi, Erfan; Pakzad, Reza; Sullman, Mark J. M.; Safiri, Saeid (Maad Rayan Publishing Company, 2018-01-01) Introduction: The objectives of this study were to provide an estimate of the prevalence of metabolic syndrome (MetS) and its components among women with PCOS; and calculate the odds ratio (OR) for MetS (using different definitions of MetS) in women with PCOS, compared to healthy controls.
Metabolic syndrome and its components in premenopausal and postmenopausal women: a comprehensive systematic review and meta-analysis on observational studies Hallajzadeh, Jamal; Khoramdad, Maliheh; Izadi, Neda; Karamzad, Nahid; Almasi-Hashiani, Amir; Ayubi, Erfan; Qorbani, Mostafa; Pakzad, Reza; Hasanzadeh, Amir; Sullman, Mark J. M.; Safiri, Saeid (Ovid Technologies (Wolters Kluwer Health), 2018-10-01) Objectives: To perform a meta-analysis on the global prevalence of metabolic syndrome (MetS) in postmenopausal women. The meta-analysis also sought to measure the relationship menopause status has with MetS and its components.

Citation Formats

E. E. Ahi et al., “Chip-based immunomagnetic separation of human chorionic gonadotropin,” Çanakkale, Turkey, 2016, p. 361, Accessed: 00, 2021. [Online]. Available: http://aacd2016.comu.edu.tr/announcements/abstract_book_of_aac.