Radiosensitization induced by zinc-doped hydroxyapatite nanoparticles in breast cancer cells

Date

2021-01-01

Author

YEDEKÇİ, FAZLI YAĞIZ
Gedik, Emre
Evis, Zafer
Dogan, Lale
ÖZYİĞİT, GÖKHAN
Gurkaynak, Murat

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Zinc-doped hydroxyapatite (HA) nanoparticles were synthesized by microwave assisted method and used with ionizing radiation for inhibition of proliferation of breast cancer cells. Zinc-doped HAs were produced in four different compositions in order to determine the best doping rate in terms of physical and biological properties. Nanoparticle characterizations were performed with X-ray diffractometer, Fourier transform infrared spectroscopy, scanning electron microscopy, and inductively coupled plasma-mass spectrometry. Viability of MDA-MB-231(isolated at M D Anderson from a pleural effusion of a patient with invasive ductal carcinoma) cells treated with nano-HA particles and radiation were assessed by MTT assay. Caspase-7 and Poly (ADP-ribose) polymerase protein expressions in samples were examined by the Western blot. X-ray diffraction patterns of our samples were found to be in good correlation with the reference HA peaks. Notably, increasing zinc amount resulted in elevated percentage of beta-tricalcium phosphate, phases. All formulations including pure HA particles were non-cytotoxic in MDA MB 231 cells. On the other hand, low rate Zn-doped HA particles showed significant anti-proliferation effect during irradiation. The combination of irradiation with Zn-doped HA particles also induced apoptosis, demonstrated as cleavage of caspase-7 and PARP proteins. In conclusion, low rate Zn-doped HA enhanced the radiation effect on breast cancer cells.

URI

https://hdl.handle.net/11511/88829

Journal

INTERNATIONAL JOURNAL OF APPLIED CERAMIC TECHNOLOGY

DOI

https://doi.org/10.1111/ijac.13707

Collections

Department of Engineering Sciences, Article

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Citation Formats

F. Y. YEDEKÇİ, E. Gedik, Z. Evis, L. Dogan, G. ÖZYİĞİT, and M. Gurkaynak, “Radiosensitization induced by zinc-doped hydroxyapatite nanoparticles in breast cancer cells,” INTERNATIONAL JOURNAL OF APPLIED CERAMIC TECHNOLOGY, pp. 0–0, 2021, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/88829.