Arabidopsis thaliana plants overexpressing the barley NICOTINAMINE SYNTHASE1 (HvNAS1) gene show tolerance to iron deficiency

Download

hseleroglu,+3974_Emre_H2.pdf

Date

2020-12-01

Author

Aksoy, Emre
MAQBOOL, AMIR
ABUDUREYİMU, BASİMUHAN

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

114
views

8
downloads

Iron (Fe) is an important trace mineral for plant development, and plants grown in Fe deficiency experience yield losses due to the leaf chlorosis. In addition to agronomic measures that can be taken to minimize these losses, new plant genotypes can be developed effectively through genetic engineering. While dicots such as Arabidopsis thaliana use a reduction-based strategy to uptake high amounts of iron from the rhizosphere, the chelation strategy has evolved in Gramineous plants including barley (Hordeum vulgare). In this study, barley NICOTIANAMINE SYNTHASE1 (HvNAS1) gene, which is responsible for the production of nicotianamine that can complex with iron, was cloned and expressed at a constitutive high level in Arabidopsis plants. The expression levels of Arabidopsis genes encoding for the proteins involved in iron uptake increased together with HvNAS1 in the T3 Arabidopsis plants. Moreover, the root lengths, root and stem fresh weights, ferric chelate reductase enzyme activities of the plants also increased in the transgenic Arabidopsis plants under Fe deficiency. In addition, significant increases in iron and zinc levels were determined in the roots and shoots of transgenic Arabidopsis plants. As a result, transgenic Arabidopsis plants overexpressing the barley HvNAS1 gene can take up more iron from the rhizosphere and carry this iron to the shoots. This study demonstrates the power of genetic engineering to develop Arabidopsis plants overexpressing the HvNAS1 gene and therefore tolerate iron deficiency.

Subject Keywords

Arabidopsis thaliana, Bbarley, Iron deficiency, Nicotianamine synthase, Tolerance

URI

https://hdl.handle.net/11511/92989

Journal

Turkish Journal of Agriculture-Food Science and Technology

DOI

https://doi.org/10.24925/turjaf.v8isp1.70-79.3974

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Arabidopsis thaliana plants overexpressing the barley NICOTINAMINE SYNTHASE1 (HvNAS1) gene show tolerance to iron deficiency Aksoy, Emre; MAQBOOL, AMIR; ABUDUREYİMU, BASİMUHAN (2020-10-04)
Arabidopsis CPL4 is an essential C-terminal domain phosphatase that suppresses xenobiotic stress responses Fukudome, Akihito; Aksoy, Emre; Wu, Xiaoqiang; Kumar, Krishna; Jeong, In Sil; May, Kimberly; Russell, William K.; Koiwa, Hisashi (2014-10-01) © 2014 The Authors.Eukaryotic gene expression is both promoted and inhibited by the reversible phosphorylation of the C-terminal domain of RNA polymerase II (pol II CTD). More than 20 Arabidopsis genes encode CTD phosphatase homologs, including four CTD phosphatase-like (CPL) family members. Although in vitro CTD phosphatase activity has been established for some CPLs, none have been shown to be involved in the phosphoregulation of pol II in vivo. Here we report that CPL4 is a CTD phosphatase essential for ...
Arabidopsis C-Terminal Domain Phosphatase-Like 1 Functions in miRNA Accumulation and DNA Methylation Jeong, In Sil; Aksoy, Emre; Fukudome, Akihito; Akhter, Salina; Hiraguri, Akihiro; Fukuhara, Toshiyuki; Bahk, Jeong Dong; Koiwa, Hisashi (2013-09-01) Arabidopsis CTD-PHOSPHATASE-LIKE 1 (CPL1) is a protein phosphatase that can dephosphorylate RNA polymerase II C-terminal domain (CTD). Unlike typical CTD-phosphatases, CPL1 contains a double-stranded (ds) RNA-binding motif (dsRBM) and has been implicated for gene regulation mediated by dsRNA-dependent pathways. We investigated the role of CPL1 and its dsRBMs in various gene silencing pathways. Genetic interaction analyses revealed that cpl1 was able to partially suppress transcriptional gene silencing and D...
Avicel-adsorbable endoglucanase production by the thermophilic fungus Scytalidium thermophilum type culture Torula thermophila Arifoglu, N; Ögel, Zümrüt Begüm (Elsevier BV, 2000-11-01) Scytalidium thermophilum type culture Torula thermophila was isolated from mushroom compost and the total cellulase, endoglucanase, Avicel-adsorbable endoglucanase activities, as well as the fungal biomass generation and cellulose utilisation were analyzed in shake flask cultures with Avicel (microcrystalline cellulose) as the carbon source. Results were compared with an industrial strain of Scyalidium thermophilum type culture Humicola insolens. The pH and temperature optima for endoglucanase activities du...
Hemicellulose coating as a substitute of sulfuring for apricot drying Übeyitoğulları, Ali; Çekmecelioğlu, Deniz; Department of Food Engineering (2014) Dry apricot is one of the most exported fruits in Turkey. However, there are some quality problems faced with sulfuring before drying. Sulfuring is a chemical method that is used to prevent/delay oxidation and fungal-bacterial growth. Indeed, sulfuring is harmful to human health. Therefore, alternatively to sulfuring, hemicellulose coating was investigated. In this study, hemicellulose was extracted from hazelnut shells by using alkaline peroxide solutions. The extraction conditions were optimized with resp...

Citation Formats

E. Aksoy, A. MAQBOOL, and B. ABUDUREYİMU, “Arabidopsis thaliana plants overexpressing the barley NICOTINAMINE SYNTHASE1 (HvNAS1) gene show tolerance to iron deficiency,” Turkish Journal of Agriculture-Food Science and Technology, vol. 8, no. sp1, pp. 70–79, 2020, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/92989.